Shaziling pig,a Chinese indigenous breed,has been classified as a fatty pig model.However,the gut microbial development and role in lipid metabolism in Shaziling pigs has been rarely reported.Here,we compared the lipid metabolic and microbial profiles at 30,60,90,150,210,and 300 d of age between Shaziling and Yorkshire pigs.Predictably,there were marked differences in the liver lipids(i.e.,choles-terol,glucose,and low-density lipoprotein)and the lipid related expressions(i.e.,SREBP1/2,LXRα/β,DGAT1/2,and FABP1-3)between Shaziling and Yorkshire pigs.Bacteria sequencing in the ileal digesta and mucosa showed that Shaziling pigs had a higher α-diversity and higher abundances of probiotics,such as Lactobacillus johnsonii,Lactobacillus amylovorus,and Clostridium butyricum.Thirty-five differentiated metabolites were further identified in the mucosa between Shaziling and Yorkshire pigs,which were enriched in the carbohydrate,protein,glucose and amino acid metabolism and bile acid biosynthesis.Furthermore,7 differentiated microbial species were markedly correlated with metabolites,indicating the role of gut microbiota in the host metabolism.Next,the role of differentiated L.johnsonii in lipid metabolism was validated in Duroc×Landrace×Yorkshire(DLY)pigs and the results showed that L.johnsonii mono-colonization promoted lipid deposition and metabolism by altering gut microbiota(i.e.,Megasphaera elsdenii and L johnsonii)and DGAT1/DGAT2/CD36-PPARy gene expressions.In conclusion,Shaziling pigs exhibited different metabolic and microbial profiles compared with Yorkshire pigs,which might have contributed to the diverse metabolic phenotypes,and the significant enrichment of L.johnsonii in Shaziling pigs promoted lipid metabolism and obesity of DLY pigs,which provided a novel idea to improve the fat content of lean pigs.

Jie Ma;Yehui Duan;Rui Li;Xiaoxiao Liang;Tiejun Li;Xingguo Huang;Yulong Yin;Jie Yin

Animal Nutritional Genome and Germplasm Innovation Research Center,College of Animal Science and Technology,Hunan Agricultural University,Changsha,410128,China;Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,410125,China;Henan Ground Biological Science&Technology Co.,Ltd.,China

动物营养（英文）

[1]Jie Ma;Yehui Duan;Rui Li;Xiaoxiao Liang;Tiejun Li;Xingguo Huang;Yulong Yin;Jie Yin-.Gut microbial profiles and the role in lipid metabolism in Shaziling pigs)[J].动物营养（英文）,2022(02):345-356

Shaziling,Predictably,digesta,amylovorus,elsdenii

Gut,microbial,profiles,role,metabolism,pigs,Chinese,indigenous,breed,has,been,classified,fatty,model,However,gut,development,rarely,reported,Here,compared,metabolic,age,between,Yorkshire,there,were,differences,liver,lipids,choles,terol,glucose,low,density,lipoprotein,expressions,SREBP1,LXR,DGAT1,FABP1,Bacteria,sequencing,ileal,mucosa,showed,that,had,higher,diversity,abundances,probiotics,such,Lactobacillus,johnsonii,Clostridium,butyricum,Thirty,five,differentiated,metabolites,further,identified,which,enriched,carbohydrate,amino,acid,bile,biosynthesis,Furthermore,species,markedly,correlated,indicating,microbiota,host,Next,was,validated,Duroc,Landrace,DLY,results,mono,colonization,promoted,deposition,by,altering,Megasphaera,DGAT2,CD36,PPARy,gene,In,conclusion,exhibited,might,have,contributed,diverse,phenotypes,significant,enrichment,obesity,provided,novel,idea,improve,content,lean

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