The gut microbiome has great effects on the digestion,absorption,and metabolism of lipids.However,the microbiota composition that can alter the fat deposition and the meat quality of pigs remains unclear.Here,we used Laiwu(LW)pigs(a native Chinese breed with higher intramuscular fat)compared with commercial crossbreed Duroc x(Landrace x Yorkshire)(DLY)pigs to investigate the effects of microbiota on meat quality,especially in intramuscular fat content.A total of 32 DLY piglets were randomly allotted to 4 groups and transplanted with fecal microbiota from healthy LW pigs.The results indicated that the high dose of fecal microbiota transplantation(HFMT)selectively enhanced fat deposition in longissimus dorsi(P＜0.05)but decreased backfat thickness(P＜0.05)compared with control group.HFMT signif-icantly altered meat color and increased feed conversation ratio(P＜0.05).Furthermore,the multi-omics analysis revealed that Bacteroides uniformis,Sphaerochaeta globosa,Hydrogenoanaerobacterium saccha-rovorans,and Pyramidobacter piscolens are the core species which can regulate lipid deposition.A total of 140 male SPF C57BL/6j mice were randomly allotted into 7 groups and administrated with these 4 mi-crobes alone or consortium to validate the relationships between microbiota and lipid deposition.Inoculating the bacterial consortium into mice increased intramuscular fat content(P＜0.05)compared with control mice.Increased expressions of lipogenesis-associated genes including cluster of differen-tiation 36(Cd36),diacylglycerol O-acyltransferase 2(Dgat2),and fatty acid synthase(FASN)were observed in skeletal muscle in the mice with mixed bacteria compared with control mice.Together,our results suggest that the gut microbiota may play an important role in regulating the lipid deposition in the muscle of pigs and mice.

Chunlin Xie;Junyong Teng;Xinkai Wang;Baoyang Xu;Yaorong Niu;Libao Ma;Xianghua Yan

State Key Laboratory of Agricultural Microbiology,College of Animal Sciences and Technology,Huazhong Agricultural University,Wuhan,Hubei,430070,China;Hubei Hongshan Laboratory,Wuhan,Hubei,430070,China;The Cooperative Innovation Center of Sustainable Pig Production,Wuhan,Hubei,430070,China;Hubei Provincial Engineering Laboratory for Pig Precision Feeding and Feed Safety Technology,Wuhan,Hubei,430070,China

动物营养（英文）

[1]Chunlin Xie;Junyong Teng;Xinkai Wang;Baoyang Xu;Yaorong Niu;Libao Ma;Xianghua Yan-.Multi-omics analysis reveals gut microbiota-induced intramuscular fat deposition via regulating expression of lipogenesis-associated genes)[J].动物营养（英文）,2022(02):84-99

Laiwu,crossbreed,HFMT,Hydrogenoanaerobacterium,saccha,rovorans,Pyramidobacter,piscolens,crobes,Inoculating,Cd36,Dgat2

Multi,omics,analysis,reveals,gut,microbiota,induced,intramuscular,deposition,via,regulating,lipogenesis,associated,microbiome,great,effects,digestion,absorption,metabolism,lipids,However,composition,that,meat,quality,pigs,remains,unclear,Here,used,LW,native,Chinese,higher,compared,commercial,Duroc,Landrace,Yorkshire,DLY,investigate,especially,content,total,piglets,were,randomly,allotted,groups,transplanted,fecal,from,healthy,results,indicated,dose,transplantation,selectively,enhanced,longissimus,dorsi,but,decreased,backfat,thickness,control,signif,icantly,altered,color,increased,feed,conversation,ratio,Furthermore,multi,revealed,Bacteroides,uniformis,Sphaerochaeta,globosa,core,species,which,regulate,male,SPF,C57BL,6j,mice,into,administrated,these,alone,consortium,validate,relationships,between,bacterial,Increased,expressions,including,cluster,differen,tiation,diacylglycerol,acyltransferase,fatty,acid,synthase,FASN,observed,skeletal,muscle,mixed,Together,our,suggest,may,play,important,role

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