Heat stress(HS)can be detrimental to the gut health of swine.Many negative outcomes induced by HS are increasingly recognized as including modulation of intestinal microbiota.In turn,the intestinal microbiota is a unique ecosystem playing a critical role in mediating the host stress response.Therefore,we aimed to characterize gut microbiota of pigs'exposure to short-term HS,to explore a possible link between the intestinal microbiota and HS-related changes,including serum cytokines,oxidation status,and intestinal epithelial barrier function.Our findings showed that HS led to intestinal morphological and integrity changes(villus height,serum diamine oxidase[DAO],serum D-lactate and the relative expressions of tight junction proteins),reduction of serum cytokines(interleukin[IL]-8,IL-12,interferon-gamma[IFN-y]),and antioxidant activity(higher glutathione[GSH]and malondialdehyde[MDA]con-tent,and lower superoxide dismutase[SOD]).Also,16S rRNA sequencing analysis revealed that although there was no difference in microbial α-diversity,some HS-associated composition differences were revealed in the ileum and cecum,which partly led to an imbalance in the production of short-chain fatty acids including propionate acid and valerate acid.Relevance networks revealed that HS-derived changes in bacterial genera and microbial metabolites,such as Chlamydia,Lactobacillus,Succinivibrio,Bifido-bacterium,Lachnoclostridium,and propionic acid,were correlated with oxidative stress,intestinal barrier dysfunction,and inflammation in pigs.Collectively,our observations suggest that intestinal damage induced by HS is probably partly related to the gut microbiota dysbiosis,though the underlying mech-anism remains to be fully elucidated.

Bing Xia;Weida Wu;Wei Fang;Xiaobin Wen;Jingjing Xie;Hongfu Zhang

State Key Laboratory of Animal Nutrition,Institute of Animal Sciences,Chinese Academy of Agricultural Sciences,Beijing,100193,China;Institute of Quality Standard and Testing Technology for Agro-Products,Key Laboratory of Agro-Product Quality and Safety,Chinese Academy of Agricultural Sciences,Beijing,100081,China;Academy of State Administration of Grain,Beijing,100037,China

动物营养（英文）

[1]Bing Xia;Weida Wu;Wei Fang;Xiaobin Wen;Jingjing Xie;Hongfu Zhang-.Heat stress-induced mucosal barrier dysfunction is potentially associated with gut microbiota dysbiosis in pigs)[J].动物营养（英文）,2022(01):289-299

valerate,Bifido

Heat,stress,induced,mucosal,barrier,dysfunction,potentially,associated,gut,microbiota,dysbiosis,pigs,HS,can,detrimental,health,swine,Many,negative,outcomes,by,are,increasingly,recognized,including,modulation,intestinal,In,turn,unique,ecosystem,playing,critical,role,mediating,host,response,Therefore,aimed,characterize,exposure,short,term,explore,possible,link,between,changes,serum,cytokines,oxidation,status,epithelial,Our,findings,showed,that,morphological,integrity,villus,height,diamine,oxidase,DAO,lactate,relative,expressions,tight,junction,proteins,reduction,interleukin,interferon,gamma,IFN,antioxidant,activity,higher,glutathione,GSH,malondialdehyde,con,lower,superoxide,dismutase,Also,16S,rRNA,sequencing,analysis,revealed,although,there,was,microbial,diversity,some,composition,differences,were,ileum,cecum,which,partly,imbalance,production,chain,fatty,acids,propionate,Relevance,networks,derived,bacterial,genera,metabolites,such,Chlamydia,Lactobacillus,Succinivibrio,bacterium,Lachnoclostridium,propionic,correlated,oxidative,inflammation,Collectively,our,observations,suggest,damage,probably,underlying,mech,anism,remains,fully,elucidated

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