典型文献
Nuclear peripheral chromatin-lamin B1 interaction is required for global integrity of chromatin architecture and dynamics in human cells
文献摘要：
The eukaryotic genome is folded into higher-order con-formation accompanied with constrained dynamics for coordinated genome functions.However,the molecular machinery underlying these hierarchically organized three-dimensional(3D)chromatin architecture and dynamics remains poorly understood.Here by combin-ing imaging and sequencing,we studied the role of lamin B1 in chromatin architecture and dynamics.We found that lamin B1 depletion leads to detachment of lamina-associated domains(LADs)from the nuclear periphery accompanied with global chromatin redistri-bution and decompaction.Consequently,the inter-chromosomal as well as inter-compartment interactions are increased,but the structure of topologically asso-ciating domains(TADs)is not affected.Using live-cell genomic loci tracking,we further proved that depletion of lamin B1 leads to increased chromatin dynamics,owing to chromatin decompaction and redistribution toward nucleoplasm.Taken together,our data suggest that lamin B1 and chromatin interactions at the nuclear periphery promote LAD maintenance,chromatin com-paction,genomic compartmentalization into chromo-some territories and A/B compartments and confine chromatin dynamics,supporting their crucial roles in chromatin higher-order structure and chromatin dynamics.
文献关键词：
中图分类号：
作者姓名：
Lei Chang;Mengfan Li;Shipeng Shao;Chen Li;Shanshan Ai;Boxin Xue;Yingping Hou;Yiwen Zhang;Ruifeng Li;Xiaoying Fan;Aibin He;Cheng Li;Yujie Sun
作者机构：
State Key Laboratory of Membrane Biology,School of Life Sciences,and Biomedical Pioneering Innovation Center(BIOPIC),Peking University,Beijing 100871,China;Bioland Laboratory(Guangzhou Regenerative Medicine and Health Guangdong Laboratory),Guangzhou 510530,China;Peking-Tsinghua Center for Life Sciences,Academy for Advanced Interdisciplinary Studies,Peking University,Beijing 100871,China;Center for Bioinformatics,School of Life Sciences,Peking University,Beijing 100871,China;Institute of Molecular Medicine,Beijing Key Laboratory of Cardiometabolic Molecular Medicine,Peking University,Beijing 100871,China;Center for Statistical Science,Peking University,Beijing 100871,China
文献出处：
引用格式：
[1]Lei Chang;Mengfan Li;Shipeng Shao;Chen Li;Shanshan Ai;Boxin Xue;Yingping Hou;Yiwen Zhang;Ruifeng Li;Xiaoying Fan;Aibin He;Cheng Li;Yujie Sun-.Nuclear peripheral chromatin-lamin B1 interaction is required for global integrity of chromatin architecture and dynamics in human cells)[J].蛋白质与细胞,2022(04):258-280
A类：
LADs,decompaction,ciating,nucleoplasm
B类：
Nuclear,peripheral,chromatin,B1,required,global,integrity,architecture,dynamics,human,cells,eukaryotic,genome,folded,into,higher,order,formation,accompanied,constrained,coordinated,functions,However,molecular,machinery,underlying,these,hierarchically,organized,three,dimensional,remains,poorly,understood,Here,by,combin,imaging,sequencing,studied,We,found,that,depletion,leads,detachment,lamina,associated,domains,from,nuclear,periphery,Consequently,chromosomal,well,interactions,are,increased,structure,topologically,TADs,not,affected,Using,live,genomic,loci,tracking,further,proved,owing,redistribution,toward,Taken,together,our,data,suggest,promote,maintenance,compartmentalization,some,territories,compartments,confine,supporting,their,crucial,roles
AB值：
0.484034
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