典型文献
Dynamic nucleosome organization after fertilization reveals regulatory factors for mouse zygotic genome activation
文献摘要：
Chromatin remodeling is essential for epigenome reprogramming after fertilization.However,the underlying mechanisms of chromatin remodeling remain to be explored.Here,we investigated the dynamic changes in nucleosome occupancy and positioning in pronucleus-stage zygotes using ultra low-input MNase-seq.We observed distinct features of inheritance and reconstruction of nucleosome positioning in both paternal and maternal genomes.Genome-wide de novo nucleosome occupancy in the paternal genome was observed as early as 1 h after the injection of sperm into ooplasm.The nucleosome positioning pattern was continually rebuilt to form nucleosome-depleted regions(NDRs)at promoters and transcription factor(TF)binding sites with differential dynamics in paternal and maternal genomes.NDRs formed more quickly on the promoters of genes involved in zygotic genome activation(ZGA),and this formation is closely linked to histone acetylation,but not transcription elongation or DNA replication.Importantly,we found that NDR establishment on the binding motifs of specific TFs might be associated with their potential pioneer functions in ZGA.Further investigations suggested that the predicted factors MLX and RFX1 played important roles in regulating minor and major ZGA,respectively.Our data not only elucidate the nucleosome positioning dynamics in both male and female pronuclei following fertilization,but also provide an efficient method for identifying key transcription regulators during development.
文献关键词：
中图分类号：
作者姓名：
Chenfei Wang;Chuan Chen;Xiaoyu Liu;Chong Li;Qiu Wu;Xiaolan Chen;Lingyue Yang;Xiaochen Kou;Yanhong Zhao;Hong Wang;Yawei Gao;Yong Zhang;Shaorong Gao
作者机构：
Institute for Regenerative Medicine,Shanghai East Hospital,Shanghai Key Laboratory of Signaling and Disease Research,Frontier Science Center for Stem Cell Research,School of Life Sciences and Technology,Tongji University,Shanghai,China;Department of Urology,Tongji Hospital,Frontier Science Center for Stem Cells,School of Life Science and Technology,Tongji University,Shanghai,China;Women's Hospital,Zhejiang University School of Medicine,Hangzhou,Zhejiang,China;Shanghai Institute of Stem Cell Research and Clinical Translation,Shanghai,China;Clinical and Translation Research Center of Shanghai First Maternity & Infant Hospital,Shanghai Key Laboratory of Signaling and Disease Research,Frontier Science Center for Stem Cell Research,School of Life Sciences and Technology,Tongji University,Shanghai,China
文献出处：
引用格式：
[1]Chenfei Wang;Chuan Chen;Xiaoyu Liu;Chong Li;Qiu Wu;Xiaolan Chen;Lingyue Yang;Xiaochen Kou;Yanhong Zhao;Hong Wang;Yawei Gao;Yong Zhang;Shaorong Gao-.Dynamic nucleosome organization after fertilization reveals regulatory factors for mouse zygotic genome activation)[J].细胞研究（英文版）,2022(09):801-813
A类：
ooplasm,rebuilt,NDRs,MLX
B类：
Dynamic,nucleosome,organization,after,fertilization,reveals,regulatory,factors,mouse,zygotic,activation,Chromatin,remodeling,essential,epigenome,reprogramming,However,underlying,mechanisms,chromatin,remain,be,explored,Here,investigated,changes,occupancy,positioning,pronucleus,stage,zygotes,using,ultra,input,MNase,seq,We,observed,distinct,features,inheritance,reconstruction,both,paternal,maternal,genomes,Genome,wide,novo,was,early,injection,sperm,into,pattern,continually,depleted,regions,promoters,transcription,binding,sites,differential,dynamics,formed,more,quickly,genes,involved,ZGA,this,formation,closely,linked,histone,acetylation,but,not,elongation,replication,Importantly,found,that,establishment,motifs,specific,TFs,might,associated,their,potential,pioneer,functions,Further,investigations,suggested,predicted,RFX1,played,important,roles,regulating,minor,major,respectively,Our,data,only,elucidate,female,pronuclei,following,also,provide,efficient,method,identifying,key,regulators,during,development
AB值：
0.584608
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