典型文献
Single-cell epigenome analysis reveals age-associated decay of heterochromatin domains in excitatory neurons in the mouse brain
文献摘要：
Loss of heterochromatin has been implicated as a cause of pre-mature aging and age-associated decline in organ functions in mammals;however,the specific cell types and gene loci affected by this type of epigenetic change have remained unclear.To address this knowledge gap,we probed chromatin accessibility at single-cell resolution in the brains,hearts,skeletal muscles,and bone marrows from young,middle-aged,and old mice,and assessed age-associated changes at 353,126 candidate cis-regulatory elements(cCREs)across 32 major cell types.Unexpectedly,we detected increased chromatin accessibility within specific heterochromatin domains in old mouse excitatory neurons.The gain of chromatin accessibility at these genomic loci was accompanied by the cell-type-specific loss of heterochromatin and activation of LINE1 elements.Immunostaining further confirmed the loss of the heterochromatin mark H3K9me3 in the excitatory neurons but not in inhibitory neurons or glial cells.Our results reveal the cell-type-specific changes in chromatin landscapes in old mice and shed light on the scope of heterochromatin loss in mammalian aging.
文献关键词：
中图分类号：
作者姓名：
Yanxiao Zhang;Maria Luisa Amaral;Chenxu Zhu;Steven Francis Grieco;Xiaomeng Hou;Lin Lin;Justin Buchanan;Liqi Tong;Sebastian Preissl;Xiangmin Xu;Bing Ren
作者机构：
Ludwig Institute for Cancer Research,La Jolla,CA,USA;School of Life Sciences,Westlake University,Hangzhou,China;Bioinformatics and Systems Biology Graduate Program,University of California San Diego,La Jolla,CA,USA;Department of Anatomy and Neurobiology,School of Medicine,University of California,Irvine,CA,USA;Center for Epigenomics,University of California San Diego,La Jolla,CA,USA.6Institute of Experimental and Clinical Pharmacology and Toxicology,Faculty of Medicine,University of Freiburg,Freiburg,Germany;The Center for Neural Circuit Mapping,University of California,Irvine,CA,USA;Department of Cellular and Molecular Medicine,University of California San Diego School of Medicine,La Jolla,CA,USA;institute for Genomic Medicine,University of California San Diego,La Jolla,CA,USA
文献出处：
引用格式：
[1]Yanxiao Zhang;Maria Luisa Amaral;Chenxu Zhu;Steven Francis Grieco;Xiaomeng Hou;Lin Lin;Justin Buchanan;Liqi Tong;Sebastian Preissl;Xiangmin Xu;Bing Ren-.Single-cell epigenome analysis reveals age-associated decay of heterochromatin domains in excitatory neurons in the mouse brain)[J].细胞研究（英文版）,2022(11):1008-1021
A类：
marrows,cCREs,LINE1
B类：
Single,epigenome,analysis,reveals,associated,decay,heterochromatin,domains,excitatory,neurons,mouse,Loss,has,been,implicated,cause,pre,mature,aging,decline,organ,functions,mammals,however,specific,types,loci,affected,by,this,epigenetic,have,remained,unclear,To,address,knowledge,gap,probed,accessibility,single,resolution,brains,hearts,skeletal,muscles,bone,from,young,middle,aged,old,mice,assessed,changes,candidate,cis,regulatory,elements,across,major,Unexpectedly,detected,increased,within,gain,these,genomic,was,accompanied,loss,activation,Immunostaining,further,confirmed,mark,H3K9me3,but,not,inhibitory,glial,cells,Our,results,landscapes,shed,light,scope,mammalian
AB值：
0.537501
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