The regeneration of the injured nerve and recovery of its function have brought attention in the medical field.Electrical stimulation(ES)can enhance the cellular biological behavior and has been widely stud-ied in the treatment of neurological diseases.Microfluidic technology can provide a cell culture platform with the well-controlled environment.Here a novel microfluidic/microelectrode composite microdevice was developed by embedding the microelectrodes to the microfluidic platform,in which microfluidics provided a controlled cell culture platform,and ES promoted the NSCs proliferation.We performed ES on rat neural stem cells(NSCs)to observe the effect on their growth,differentiation,proliferation,and pre-liminary explored the ES influence on cells in vitro.The results of immunofluorescence showed that ES had no significant effect on the NSCs specific expression,and the NSCs specific expression reached 98.9％±0.4％after three days of ES.In addition,ES significantly promoted cell growth and the cell prolifera-tion rate reached 49.41％.To conclude,the microfluidic/microelectrode composite microdevice can play a positive role in the nerve injury repair and fundamental research of neurological diseases.

Qian Li;Bodong Kang;Libin Wang;Tao Chen;Yu Zhao;Shilun Feng;Rongjing Li;Hongtian Zhang

Institute of Advanced Photonics Technology,Faculty of Materials and Manufacturing,Beijing University of Technology,Beijing 100124,China;Key Laboratory of Trans-scale Laser Manufacturing Technology(Beijing University of Technology),Ministry of Education,Beijing 100124,China;State Key Laboratory of Transducer Technology,Shanghai Institute of Microsystem and Information Technology,Chinese Academy of Sciences,Shanghai 200050,China;Department of Neurosurgery,The 7th Medical Center,General Hospital of the Chinese People's Liberation Army,Beijing 100700,China

中国化学快报（英文版）

[1]Qian Li;Bodong Kang;Libin Wang;Tao Chen;Yu Zhao;Shilun Feng;Rongjing Li;Hongtian Zhang-.Microfluidics embedded with microelectrodes for electrostimulation of neural stem cells proliferation)[J].中国化学快报（英文版）,2022(03):1308-1312

electrostimulation,microdevice

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