Excessive reactive oxygen species (ROS) can cause oxidative damage and lead to various metabolic disease. Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) is a new kind of protein-rich functional food, the protein in which has been proved to have good antioxidant capacity. In this study, in order to further explore the antioxidant mechanism of Tartary buckwheat protein, 4 peptides (CR-8, LR-8, GK-10 and SR-12) were isolated and identified from it. H2O2 was used to induce oxidative damage to Caco-2 cells to evaluate antioxidant capacity of these peptides. The results of superoxide dismutase (SOD), total antioxidant capacity (T-AOC) and mitochondrial membrane potential etc. showed that these peptides have superior antioxidant capacity. CR-8 has the best antioxidant capacity. In order to further clarify the antioxidant mechanism of CR-8, metabolomics was used to analyze related metabolites and metabolic pathways. The results showed that after CR-8 intervention, the content of metabolites such as L-acetyl carnitine has increased. This indicated that CR-8 can improve the antioxidant capacity of damaged cells by intervening in multiple metabolic pathways. This also revealed the anti-oxidant mechanism of tartary buckwheat protein. In conclusion, it provided a theoretical basis for further studying the activity of tartary buckwheat portein and utilizing buckwheat resources.

School of Perfume and Aroma Technology,Shanghai Institute of Technology,Shanghai 201418,China;University Think Tank of Shanghai Municipality,Institute of Beautiful China and Ecological Civilization,Shanghai 201418,China

[1]Yiming Zhou;Xuanming She;Zhidong Chen;Yun Wei;Ying Xiao;Xiaoli Zhou-.Tartary buckwheat (Fagopyrum tataricum (L.) Gaertn) protein-derived antioxidant peptides: mechanisms of action and structure-activity relationship in Caco-2 cell models)[J].食品科学与人类健康（英文）,2022(06):1580-1590

Tartary,buckwheat,tartary,portein

Fagopyrum,tataricum,Gaertn,protein,derived,antioxidant,peptides,mechanisms,action,structure,activity,relationship,Caco,models,Excessive,reactive,oxygen,species,ROS,can,cause,oxidative,lead,various,metabolic,disease,new,kind,rich,functional,food,which,has,been,proved,have,good,capacity,In,this,order,further,explore,CR,LR,GK,SR,were,isolated,identified,from,H2O2,was,used,induce,cells,evaluate,these,results,superoxide,dismutase,total,AOC,mitochondrial,membrane,potential,etc,showed,that,superior,best,clarify,metabolomics,analyze,related,metabolites,pathways,after,intervention,content,such,acetyl,carnitine,increased,This,indicated,improve,damaged,by,intervening,multiple,also,revealed,conclusion,provided,theoretical,basis,studying,utilizing,resources

0.479158