OBJECTIVE:To study the molecular mechanism of Buyang Huanwu Tang(补N阳还五汤)(BHT)protecting retinal ganglion cells(RGCs)from oxygen induced oxidative stress and apoptosis after anterior ischemia.METHODS:In this study,the Chinese herbs of BHT were extracted by first boiling in water,then were filtered,concentrated,and freeze-dried.The chemical profile of BHT extract was determined by liquid chromatography mass spectrometry(LC-MS).H2O2-induced RGC-5 cells were used as a cell model to investigate the protective effect and mechanism of BHT on RGCs.RESULTS:The survival rate of damaged RGC-5 by BHT was significantly increased by the 3-(4,5)-dimethylthiahiazo(-z-y1)-3,5-di-phenytetrazolium-romid method.Fluorescence activating cell sorter(FACS analysis)showed that BHT could significantly reduce apoptosis induced by oxidative stress via the reactive oxygen species(ROS)-mitogen-activated protein kinase(MAPK)-Caspase-3 signal pathway.CONCLUSION:BHT possesses a high antioxidant capacity and could significantly reduce ROS levels of RGC-5 cells damaged by H2O2.Therefore,the present study has provided possible alternative strategies for the prevention and treatment of ischemic optic disease by using traditional Chinese herbal formulas.

ZHENG Wei;WANG Mingxing;LIU Shanxue;LUAN Chao;ZHANG Yanqiu;XU Duoduo;WANG Jian

Department of Ophthalmology,First Affiliated Hospital,Changchun University of Chinese Medicine,Changchun 130021,China;Department of Experimental Center,First Affiliated Hospital,Changchun University of Chinese Medicine,Changchun 130021,China;Changchun University of Chinese Medicine,Changchun 130117,China;Pharmaceutical Department,First Affiliated Hospital,Changchun University of Chinese Medicine,Changchun 130021,China;College of Pharmacy,Changchun University of Chinese Medicine,Changchun 130117,China;Department of Encephalopathy Center,First Affiliated Hospital,Changchun University of Chinese Medicine,Changchun 130021,China

中医杂志（英文版）

[1]ZHENG Wei;WANG Mingxing;LIU Shanxue;LUAN Chao;ZHANG Yanqiu;XU Duoduo;WANG Jian-.Buyang Huanwu Tang(补阳还五汤)protects H202-induced RGC-5 cell against oxidative stress and apoptosis via reactive oxygen species-mitogen-activated protein kinase signaling pathway)[J].中医杂志（英文版）,2022(06):885-891

phenytetrazolium,romid,sorter

Buyang,Huanwu,Tang,补阳还五汤,protects,H202,induced,against,oxidative,stress,apoptosis,via,reactive,oxygen,species,mitogen,activated,protein,kinase,signaling,pathway,OBJECTIVE,To,study,molecular,mechanism,BHT,protecting,retinal,ganglion,cells,RGCs,from,after,anterior,ischemia,METHODS,In,this,Chinese,herbs,were,extracted,by,first,boiling,water,then,filtered,concentrated,freeze,dried,chemical,profile,was,determined,liquid,chromatography,mass,spectrometry,LC,H2O2,used,model,investigate,protective,effect,RESULTS,survival,damaged,significantly,increased,dimethylthiahiazo,y1,method,Fluorescence,activating,FACS,analysis,showed,that,could,reduce,ROS,MAPK,Caspase,CONCLUSION,possesses,high,antioxidant,capacity,levels,Therefore,present,has,provided,possible,alternative,strategies,prevention,treatment,ischemic,optic,disease,using,traditional,herbal,formulas

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