Vascular endothelial cells and oxidation reduction system play an important role in the pathogenesis of atherosclerosis(AS).If these conditions are disordered,it will inevitably lead to plaque formation and even rupture.Astragaloside Ⅳ(AsⅣ)and salvianolic acid B(Sal B)are the main active ingredients of Astragalus membranaceus and Salvia miltiorrhiza,respectively,and found to ameliorate vascular endothelial dysfunction and protect against oxidative stress in recent studies.However,it is still unknown if the combination of AsⅣ and Sal B(AsⅣ+Sal B)can inhibit the development of plaque through amplifying the protective effect of vas-cular endothelial cells and anti-oxidative stress effect.To clarify the role of AsⅣ+Sal B in AS,we observed the efficacy of each group(Control,Model,AsⅣ,Sal B,and AsⅣ+Sal B)by biomolecular assays,such as observing the pathological morphology of the aorta by oil red O staining,evaluating the level of oxidative stress and endothelial cells in the serum by the Elisa test,and analyzing the changes of all small molecule metabolites in liver tissue by UPLC-QTOF-MS.Results showed that AsⅣ,Sal B and AsⅣ+Sal B de-creased the deposition of lipid in the arterial wall,so as to exert the effect of anti-oxidant stress and vascular endothelial protection,where the inhibitory effect of AsⅣ+Sal B was the most obvious.Metabonomics analysis showed that Sal B regulated the metabolic pathways of arginine and proline.AsⅣ regulated glycerol metabolism and saturated fatty acid biosynthesis metabolism.AsⅣ+Sal B is mainly related to the regulation of the citrate cycle(TCA cycle),alanine,aspartic acid,and glutamate metabolism,cysteine,and me-thionine metabolism.Succinic acid and methionine are synergistic metabolites that exert an enhancing effect when AsⅣ and Sal B were used in combination.In conclusion,we demonstrated that AsⅣ acompanied with Sal B can be successfully used for anti-oxidat-ive stress and vascular endothelial protection of AS,and succinic acid and methionine are the synergistic metabolites.

Experimental Center, Shandong University of Traditional Chinese Medicine, Jinan 250355, China;Graduate School,Liaoning University of Traditional Chinese Medicine,Shenyang 110847,China;College of Continuing Education,Shandong University of Traditional Chinese Medicine,Jinan,250355,China;Innovative Institute of Chinese Medicine and Pharmacy,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;College of Traditional Chinese Medicine,Shandong University of Traditional Chinese Medicine,Jinan 250355,China;Department of Cardiology,the Affiliated Hospital of Shandong University of Traditional Chinese Medicine,Jinan 250011,China

[1]KONG Xiang-Lin;LYU Qin;ZHANG Ya-Qi;KANG Dong-Fang;LI Chao;ZHANG Lei;GAO Zi-Chen;LIU Xin-Xin;WU Ji-Biao;LI Yun-Lun-.Effect of astragaloside Ⅳ and salvianolic acid B on antioxidant stress and vascular endothelial protection in the treatment of atherosclerosis based on metabonomics)[J].中国天然药物,2022(08):601-613

thionine,acompanied,oxidat

Effect,astragaloside,salvianolic,acid,antioxidant,stress,vascular,endothelial,protection,treatment,atherosclerosis,metabonomics,Vascular,cells,oxidation,reduction,system,play,important,role,pathogenesis,AS,If,these,conditions,are,disordered,will,inevitably,lead,plaque,formation,even,rupture,Astragaloside,active,ingredients,Astragalus,membranaceus,Salvia,miltiorrhiza,respectively,found,ameliorate,dysfunction,against,oxidative,recent,studies,However,still,unknown,combination,+Sal,can,development,through,amplifying,protective,effect,To,clarify,observed,efficacy,each,group,Control,Model,by,biomolecular,assays,such,observing,pathological,morphology,aorta,oil,staining,evaluating,level,serum,Elisa,test,analyzing,changes,small,molecule,metabolites,liver,tissue,UPLC,QTOF,Results,showed,that,creased,deposition,lipid,arterial,wall,exert,where,inhibitory,was,most,obvious,Metabonomics,analysis,regulated,metabolic,pathways,arginine,proline,glycerol,metabolism,saturated,fatty,biosynthesis,mainly,related,regulation,citrate,cycle,TCA,alanine,aspartic,glutamate,cysteine,Succinic,methionine,synergistic,enhancing,when,were,used,In,conclusion,demonstrated,be,successfully,succinic

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