Objective:Taxifolin is a natural flavonoid compound that can be isolated from onions,grapes,oranges and grapefruit.It also acts as a medicine food homology with extraordinary antioxidant and anti-inflammatory activity.This study aims to explain the protective effects and potential mechanisms of tax-ifolin against inflammatory reaction.Methods:Levels of interleukin(IL)-6,IL-1β and intracellular reactive oxygen species(ROS)were assessed in different time after the treatment of taxifolin in RAW264.7 cells induced by lipopolysaccharide(LPS).Subsequently,the mRNA and protein levels of inducible nitric oxide synthase(iNOS),vascular endothelial growth factor(VEGF),cyclooxygenase(COX)-2,tumor necrosis factor(TNF)-α and the phosphorylation expression levels of the MAPK signal pathway were also evaluated.A silico analysis was used to explain the binding situation for the investigation of taxifolin and MAPK signal pathway.And then MAPK inhibi-tors were used to reveal the expression level of iNOS,VEGF,COX-2 and TNF-α in RAW264.7 cells.Results:It was demonstrated that cell inflammatory damage induced by LPS was significantly alleviated after the treatment of taxifolin.Then,the mRNA and protein levels of iNOS,VEGF,COX-2 and TNF-α were reduced and the phosphorylation expression levels of the MAPK signal pathway were down-regulated remarkably as well.In silico analysis,taxifolin could form a relatively stable combination with MAPK sig-nal pathway.MAPK inhibitors showed increasing or decreasing effect in the mRNA levels of iNOS,VEGF,COX-2 and TNF-α,which suggesting that taxifolin down-regulated iNOS,VEGF,COX-2 and TNF-α expres-sions were not entirely through the MAPK pathway.Conclusion:This finding demonstrated that taxifolin improved the inflammatory responses that partly involved in the phosphorylation expression level of MAPK signal pathway in RAW264.7 cells exposed to acute stress.

Key Laboratory of Quality-marker of Traditional Chinese Medicines,Tianjin Institute of Pharmaceutical Research Co.,Ltd.,Tianjin 300462,China;Center for Drug Evaluation,National Medical Products Administration,Beijing 100022,China;Department of Pharmacology,Tianjin Medical University,Tianjin 300070,China;State Key Laboratory of Drug Delivery Technology and Pharmacokinetics,Tianjin Institute of Pharmaceutical Research Co.,Ltd.,Tianjin 300462,China;Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemistry Engineering and Technology,Tianjin University,Tianjin 300072,China;Pharmaceuticai Armaceuticai DA REN TANG Group Corporation Limited Traditional Chinese Pharmacy Researchinstitute,Tianjin 300457,China;Tianjin Key Laboratory of Quality Control in Chinese Medicine,Tianjin 300457,China;Research Unit for Drug Metabolism,Chinese Academy of Medical Sciences,Beijing 100730,China

[1]Xingyan Zhang;Xiaoyan Lian;Huling Li;Wenjing Zhao;Xin Li;Fujun Zhou;Yutong Zhou;Tao Cui;Yuli Wang;Changxiao Liu-.Taxifolin attenuates inflammation via suppressing MAPK signal pathway in vitro and in silico analysis)[J].中草药（英文版）,2022(04):554-562

Taxifolin,ifolin

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