典型文献
Transcriptome analysis to identify candidate genes related to chlorogenic acid biosynthesis during development of Korla fragrant pear in Xinjiang
文献摘要：
Korla fragrant pear (KFP) with special fragrance is a unique cultivar in Xinjiang, China. In order to explore the biosynthesis molecular mechanism of chlorogenic acid (CGA) in KFP, the samples at different development periods were collected for transcriptome analysis. High performance liquid chromatography analysis showed that CGA contents of KFP at 88, 118 and 163 days after full bloom were (20.96 ± 1.84), (12.01 ± 0.91) and (7.16 ± 0.41) mg/100 g, respectively, and decreased with the fruit development. Pears from these typical 3 periods were selected for de novo transcriptome assemble and 68059 unigenes were assembled from 444037960 clean reads. One 'phenylpropanoid biosynthesis' pathway including 57 unigenes, 11 PALs, 1 PTAL, 64CLs, 9 C4Hs, 25 HCTs and 5 C3'Hs related to CGA biosynthesis was determined. It was found that the expression levels of 11 differentially expressed genes including 1 PAL, 2 C4Hs, 34CLs and 5 HCTs were consistent with the change of CGA content. Quantitative polymerase chain reaction analysis further showed that 8 unigenes involved in CGA biosynthesis were consistent with the RNA-seq data. These findings will provide a comprehensive understanding and valuable information on the genetic engineering and molecular breeding in KFP.
文献关键词：
中图分类号：
作者姓名：
Hao Wen;Wenqiang Wang;Xi Jiang;Minyu Wu;Hongjin Bai;Cuiyun Wu;Lirong Shen
作者机构：
Department of Food Science and Nutrition,Zhejiang Key Laboratory for Agro-Food Processing,Zhejiang University,Hangzhou 310058,China;College of Horticulture Forestry,Tarim University of Land-Reclamation,Aral 843300,China
文献出处：
引用格式：
[1]Hao Wen;Wenqiang Wang;Xi Jiang;Minyu Wu;Hongjin Bai;Cuiyun Wu;Lirong Shen-.Transcriptome analysis to identify candidate genes related to chlorogenic acid biosynthesis during development of Korla fragrant pear in Xinjiang)[J].食品科学与人类健康（英文）,2022(04):854-864
A类：
Korla,KFP,Pears,PALs,PTAL,64CLs,C4Hs,HCTs,34CLs
B类：
Transcriptome,analysis,identify,candidate,related,chlorogenic,acid,biosynthesis,during,development,fragrant,pear,Xinjiang,special,fragrance,unique,cultivar,China,In,order,explore,molecular,mechanism,CGA,samples,periods,were,collected,transcriptome,High,performance,liquid,chromatography,showed,that,contents,days,after,full,bloom,respectively,decreased,fruit,from,these,typical,selected,novo,unigenes,assembled,clean,reads,One,phenylpropanoid,pathway,including,C3,was,determined,It,found,expression,levels,differentially,expressed,consistent,change,Quantitative,polymerase,chain,reaction,further,involved,seq,data,These,findings,will,provide,comprehensive,understanding,valuable,information,genetic,engineering,breeding
AB值：
0.467602
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