OBJECTIVE:To further clarify the anticancer mechanisms of Liujunzi decoction(六君子汤)and provide possible targets for the treatment of advanced-stage non-small cell lung cancer(NSCLC)by re-analyzing differential gene expression profile of peripheral blood mononuclear cells(PBMCs)from Liujunzi decoction-treated NSCLC patients receiving first-line chemotherapy.METHODS:The PBMC gene expression microarray data set GSE61926 was retrieved from a high throughput gene expression database.Differentially expressed genes(DEGs)were screened by paired sample t-test and the multiple ratio method.Gene ontology and Kyoto encyclopedia of genes and genomes(KEGG)pathway analyses were performed using the DAVID database.The protein-protein interaction(PPI)network was constructed using interaction gene library retrieval tools and Cytoscape software.RESULTS:A total of 162 DEGs were identified,with 67 upregulated genes and 95 downregulated genes.The functional distribution of Gene Oncology(GO)genes showed that DEGs were mostly concentrated in extracellular regions,calcium ion binding,and transcriptase activity.KEGG pathway analysis showed that cytokine-cytokine receptor interactions were significantly enriched.PPI network analysis screened out the top 10 central protein-coding genes with the highest nodal degree:IL2,PIWIL4,DICER1,PIWIL2,SAA1,XCL1,IL22RA1,ARHGAP11A,DCP1A,and GDNF.Among them,the central protein-coding gene with the highest node degree was IL2.In addition,the central protein-coding genes with high node degrees and high molecular complex detection(MCODE)scores were PIWIL4,DICER1,PIWIL2,and DCP1A,all of which are related to tumor development.CONCLUSIONS:One signaling pathway and 10 central protein-coding genes related to anticancer mechanisms were screened by re-analysis of GSE61926 data.IL2,PIWIL4,DICER1,PIWIL2,and DCP1A may have important roles in the mechanism of Liujunzi decoction treatment against NSCLC.Our results suggest that the anticancer mechanism of Liujunzi decoction may be related to gene silencing by RNA and the biological processes of piwi-interacting RNA and other small RNAs.

XIN Xiaoli;WANG Guodong;HAN Ru;JIANG Yi;LIU Chang;LIU Lingshuang;XU Zhenye

Department of Oncology,Longhua Hospital affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 200032,China;Department of Orthopedics,Longhua Hospital affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai 20032,China;Department of human resources,Shandong Academy of Occupational Health and Occupational Medicine,Jinan 250000,China

中医杂志（英文版）

[1]XIN Xiaoli;WANG Guodong;HAN Ru;JIANG Yi;LIU Chang;LIU Lingshuang;XU Zhenye-.Mechanism underlying the effect of Liujunzi decoction(六君子汤)on advanced-stage non-small cell lung cancer in patients after first-line chemotherapy)[J].中医杂志（英文版）,2022(01):108-115

Liujunzi,GSE61926,XCL1,IL22RA1,ARHGAP11A,DCP1A,piwi

Mechanism,underlying,effect,decoction,六君子汤,advanced,stage,small,lung,patients,after,first,line,chemotherapy,OBJECTIVE,To,further,clarify,anticancer,mechanisms,provide,possible,targets,treatment,NSCLC,by,analyzing,differential,expression,profile,peripheral,blood,mononuclear,cells,PBMCs,from,treated,receiving,METHODS,microarray,set,was,retrieved,throughput,database,Differentially,expressed,genes,DEGs,were,screened,paired,sample,test,multiple,ratio,method,Gene,ontology,Kyoto,encyclopedia,genomes,pathway,analyses,performed,using,DAVID,protein,PPI,network,constructed,library,retrieval,tools,Cytoscape,software,RESULTS,total,identified,upregulated,downregulated,functional,distribution,Oncology,showed,that,mostly,concentrated,extracellular,regions,calcium,binding,transcriptase,activity,analysis,cytokine,receptor,interactions,significantly,enriched,out,top,central,coding,highest,nodal,PIWIL4,DICER1,PIWIL2,SAA1,GDNF,Among,them,node,In,addition,degrees,molecular,complex,detection,MCODE,scores,which,related,tumor,development,CONCLUSIONS,One,signaling,may,have,important,roles,against,Our,results,suggest,be,silencing,biological,processes,interacting,RNAs

0.467857