Intestinal epithelial homeostasis plays an important role in intestinal morphology and function.How-ever,the developmental changes in intestinal epithelial cell turnover in piglets during early weaning are unknown so far.Thus,the aim of this work was to detect changes in piglet gut development from weaning to post-weaning d 14.Accordingly,40 piglets were used in the present study,and 8 piglets were randomly selected for sampling at d 0,1,3,7 and 14 post-weaning,respectively.The results showed that weaning stress significantly affected small intestinal morphological architecture,and this impact was the worst on d 3,and then returned to normal on d 14.Furthermore,the number of the marker of prolif-eration Ki-67(Ki67)positive cells was decreased on d l and 3,and then recovered on d 14(P＜0.001).Also,weaning strikingly increased jejunal epithelial cell shedding on d l to 7 compared on d 0(P＜0.05).Moreover,weaning remarkably affected the number of small intestinal enterocytes,goblets and endo-crine cells(P＜0.05),and there were also significant differences in genes expression related to prolif-eration and differentiation(P＜0.05).Additionally,the mechanistic target of rapamycin(mTOR)phosphorylation level was higher on d 3(P＜0.05).However,the Wingless/Intl(WNT)/β-catenin pathway was not influenced by post-weaning days.Taken together,weaning induced noteworthy changes in intestinal epithelial cell proliferation,differentiation and shedding,and the mTOR signaling pathway was involved in this process.Our findings provide a cellular mechanism for intestinal devel-opmental changes during weaning periods.This may provide nutritionists with better insight into designing efficient in-feed alternatives for preventing the unfavorable gut development in weaning piglets.

Hunan International Joint Laboratory of Animal Intestinal Ecology and Health,Laboratory of Animal Nutrition and Human Health,College of Life Sciences,Hunan Normal University,Changsha 410081,Hunan,China;Hunan Provincial Key Laboratory of Animal Nutritional Physiology and Metabolic Process,Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production,Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,Hunan,China

[1]Min Wang;Lixia Wang;Xian Tan;Lei Wang;Xia Xiong;Yancan Wang;Qiye Wang;Huansheng Yang;Yulong Yin-.The developmental changes in intestinal epithelial cell proliferation,differentiation,and shedding in weaning piglets)[J].动物营养（英文）,2022(02):214-222

goblets,crine,Wingless,Intl,nutritionists

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