Previous study have demonstrated that a compound composed of water-soluable Ganoderma lucidum polysaccharides (GLP) and Polyporus umbellatus polysaccharides (PUP) in a ratio of 3 : 1 named GPP enhances innate immune function in mice through enhancing the function of macrophage cells and activity of natural killer (NK) cells. Here in our research, we further investigated the effect of GPP on the diversity and composition of intestinal flora, and explored its effect on colitis model mice. The immunoregulatory verification experiments of GPP were conducted in both normal and DSS-induced mice model. Our research showed that GPP increased the diversity of intestinal microorganisms in mice with the extension of administration time. Daily GPP intake attenuated DSS-induced colon injury, protected the splenic lymphocyte proliferation ability, enhanced the serum hemolysin synthesis, and increased peripheral phagocytes and NK cell activity in model mice. Comparisons of the predominant gene pathways of the bacterial microbiota showed that DNA repair and recombination, base mismatch repair pathways was stronger in GPP-treatment group than in control group, indicating the possible molecular mechanisms of immune function regulation. Our study showed that GPP regulated immune function in both health and colitis model, and had a positive effect on maintaining intestinal flora homeostasis.

Liyuan Li;Yannan Guo;Qing Huang;Xiaojin Shi;Qingqing Liu;Fang Wang;Qingfei Liu;Kang Yu;Zhao Wang

MOE Key Laboratory of Protein Science,School of Pharmaceutical Sciences,Tsinghua University,Beijing 100084,China;Tsinghua-Peking Center for Life Sciences,Tsinghua University,Beijing 100084,China;Tianjin Research Institute for Advanced Equipment,Tianjin 300300,China;Department of Clinical Nutrition and Department of Health Medicine,Peking Union Medical College Hospital,Chinese Academy of Medical Sciences,Beijing 100730,China

食品科学与人类健康（英文）

[1]Liyuan Li;Yannan Guo;Qing Huang;Xiaojin Shi;Qingqing Liu;Fang Wang;Qingfei Liu;Kang Yu;Zhao Wang-.GPP (composition of Ganoderma lucidum polysaccharides and Polyporus umbellatus polysaccharides) protects against DSS-induced murine colitis by enhancing immune function and regulating intestinal flora)[J].食品科学与人类健康（英文）,2022(04):795-805

Polyporus,umbellatus,soluable

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