Objective:Acute lung injury(ALI)is a serious respiratory dysfunction caused by pathogen or physical invasion.The strong induced inflammation often causes death.Tanshinone ⅡA(Tan-ⅡA)is the major con-stituent of Salvia miltiorrhiza Bunge and has been shown to display anti-inflammatory effects.The aim of the current study was to investigate the effects of Tan-ⅡA on ALI.Methods:A murine model of lipopolysaccharide(LPS)-induced ALI was used.The lungs and serum sam-ples of mice were extracted at 3 days after treatment.ALI-induced inflammatory damages were con-firmed from cytokine detections and histomorphology observations.Effects of Tan-ⅡA were investigated using in vivo and in vitro ALI models.Tan-ⅡA mechanisms were investigated by performing Western blot and flow cytometry experiments.A wound-healing assay was performed to confirm the Tan-ⅡA function.Results:The cytokine storm induced by LPS treatment was detected at 3 days after LPS treatment,and alveolar epithelial damage and lymphocyte aggregation were observed.Tan-ⅡA treatment attenuated the LPS-induced inflammation and reduced the levels of inflammatory cytokines released not only by inhibiting neutrophils,but also by macrophage.Moreover,we found that macrophage activation and polarization after LPS treatment were abrogated after applying the Tan-ⅡA treatment.An in vitro assay also confirmed that including the Tan-ⅡA supplement increased the relative amount of the M2 subtype and decreased that of M1.Rebalanced macrophages and Tan-ⅡA inhibited activations of the nuclear factor-κB and hypoxia-inducible factor pathways.Including Tan-ⅡA and macrophages also improved alveolar epithelial repair by regulating macrophage polarization.Conclusion:This study found that while an LPS-induced cytokine storm exacerbated ALI,including Tan-ⅡA could prevent ALI-induced inflammation and improve the alveolar epithelial repair,and do so by reg-ulating macrophage polarization.

Jia-yi Zhao;Jin Pu;Jian Fan;Xin-yu Feng;Jian-wen Xu;Rong Zhang;Yan Shang

Department of General Practice,Changhai Hospital,Naval Medical University,Shanghai 200433,China;Department of General Practice,Changhai Hongkou Hospital,Shanghai 200081,China;Department of Special Clinic,Changhai Hospital,Naval Medical University,Shanghai 200433,China;Shanghai Institute of Biochemical and Cell Biology,Chinese Academy of Sciences,Shanghai 200031,China;Department of Nursing,Shidong Hospital,University of Shanghai for Science and Technology,Shanghai 200438,China;Department of Respiratory and Critical Care Medicine,Changhai Hospital,Naval Medical University,Shanghai 200433,China

结合医学学报（英文版）

[1]Jia-yi Zhao;Jin Pu;Jian Fan;Xin-yu Feng;Jian-wen Xu;Rong Zhang;Yan Shang-.Tanshinone ⅡA prevents acute lung injury by regulating macrophage polarization)[J].结合医学学报（英文版）,2022(03):274-280

stituent,histomorphology,Rebalanced

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