Background:Tauroursodeoxycholic acid (TUDCA), a hydrophilic bile acid, is the main medicinal component of bear bile and is commonly used to treat a variety of hepatobiliary diseases. Meanwhile, TUDCA has been shown to modulate the intestinal barrier function and alleviate DSS-induced colitis in mice. However, the effect of TUDCA on the intestinal barrier of weaned piglets remains largely unclear. Methods:The weaned piglets and porcine IPEC-J2 intestinal epithelial cells were used to investigate the effects of TUDCA on intestinal barrier function in weaned piglets and explore the possible underlying mechanisms. In vivo, 72 healthy weaned piglets were randomly allocated into 2 groups according to their gender and body weight, and piglets were fed the basal diet with 0 (control, CON) and 200 mg/kg TUDCA for 30 d, respectively. Three female and three male piglets reflecting the average bodyweight were slaughtered in each group and samples were collected. In vitro, IPEC-J2 cells were subjected to 100μmol/L TUDCA to explore the possible underlying mechanisms. Results:Our results demonstrated that dietary TUDCA supplementation significantly reduced the diarrhea incidence of weaned piglets, possibly attributing to the TUDCA-enhanced intestinal barrier function and immunity. In addition, TUDCA supplementation altered serum metabolites and the relative abundance of certain gut bacteria, which might contribute to the improved intestinal barrier function. Furthermore, the in-vitro results showed that TUDCA improved the E. coli-induced epithelial barrier impairment of IPEC-J2 cells and increased Takeda G-coupled protein receptor 5 (TGR5) protein expression. However, knockdown of TGR5 and inhibition of myosin light chain kinase (MLCK) pathway abolished the TUDCA-improved epithelial barrier impairment in E. coli-treated IPEC-J2 cells, indicating the involvement of TGR5-MLCK in this process. Conclusions:These findings showed that TUDCA improved intestinal barrier function associated with TGR5-MLCK pathway and the alteration of serum metabolites and gut bacteria in weaned piglets, suggesting the potential application of TUDCA in improving gut health in piglet production.

Min Song;Fenglin Zhang;Yiming Fu;Xin Yi;Shengchun Feng;Zhichang Liu;Dun Deng;Qiang Yang;Miao Yu;Canjun Zhu;Xiaotong Zhu;Lina Wang;Ping Gao;Gang Shu;Xianyong Ma;Qingyan Jiang;Songbo Wang

Guangdong Provincial Key Laboratory of Animal Nutrition Control,ALLT ECH-SCAU Animal Nutrition Control Research Alliance,and National Engineering Research Center for Breeding Swine Industry,College of Animal Science,South China Agricultural University,Guangzhou 510642,P.R.China;Institute of Animal Science,Guangdong Academy of Agricultural Sciences,State Key Laboratory of Livestock and Poultry Breeding,Key Laboratory of Animal Nutrition and Feed Science in South China,Ministry of Agriculture and Rural Affairs,Guangdong Key Laboratory of Animal Breeding and Nutrition,Guangdong Engineering Technology Research Center of Animal Meat Quality and Safety Control and Evaluation,Guangzhou 510640,P.R. China;Maoming Branch,Guangdong Laboratory for Lingnan Modern Agricultural Science and Technology,Maoming 525000,P.R.China

畜牧与生物技术杂志（英文版）

[1]Min Song;Fenglin Zhang;Yiming Fu;Xin Yi;Shengchun Feng;Zhichang Liu;Dun Deng;Qiang Yang;Miao Yu;Canjun Zhu;Xiaotong Zhu;Lina Wang;Ping Gao;Gang Shu;Xianyong Ma;Qingyan Jiang;Songbo Wang-.Tauroursodeoxycholic acid (TUDCA) improves intestinal barrier function associated with TGR5-MLCK pathway and the alteration of serum metabolites and gut bacteria in weaned piglets)[J].畜牧与生物技术杂志（英文版）,2022(06):1917-1931

Tauroursodeoxycholic

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