Neural/glial antigen 2 (NG2)-expressing cells has multipotent stem cell activity under cerebral ischemia. Our study examined the effects of electroacupuncture (EA) therapy (2 Hz, 1 or 3 mA, 20 minutes) at the Sishencong acupoint on motor function after ischemic insult in the brain by investigating the rehabilitative potential of NG2-derived cells in a mouse model of ischemic stroke. EA stimulation alleviated motor deficits caused by ischemic stroke, and 1 mA EA stimulation was more efficacious than 3 mA EA stimulation or positive control treatment with edaravone, a free radical scavenger. The properties of NG2-expressing cells were altered with 1 mA EA stimulation, enhancing their survival in perilesional brain tissue via reduction of tumor necrosis factor alpha expression. EA stimulation robustly activated signaling pathways related to proliferation and survival of NG2-expressing cells and increased the expression of neurotrophic factors such as brain-derived neurotrophic factor, tumor growth factor beta, and neurotrophin 3. In the perilesional striatum, EA stimulation greatly increased the number of NG2-expressing cells double-positive for oligodendrocyte, endothelial cell, and microglia/macrophage markers (CC1, CD31, and CD68). EA therapy also greatly activated brain-derived neurotrophic factor/tropomyosin receptor kinase B and glycogen synthase kinase 3 beta signaling. Our results indicate that EA therapy may prevent functional loss at the perilesional site by enhancing survival and differentiation of NG2-expressing cells via the activation of brain-derived neurotrophic factor -induced signaling, subsequently ameliorating motor dysfunction. The animal experiments were approved by the Animal Ethics Committee of Pusan National University (approval Nos. PNU2019-2199 and PNU2019-2884) on April 8, 2019 and June 19, 2019.

Hong Ju Lee;Da Hee Jung;Nam Kwen Kim;Hwa Kyoung Shin;Byung Tae Choi

Department of Korean Medical Science,School of Korean Medicine,Pusan National University,Yangsan,Republic of Korea;Graduate Training Program of Korean Medicine for Healthy Aging,Pusan National University,Yangsan,Republic of Korea;Department of Korean Ophthalmology,Otolaryngology and Dermatology,School of Korean Medicine,Pusan National University,Yangsan,Republic of Korea

中国神经再生研究（英文版）

[1]Hong Ju Lee;Da Hee Jung;Nam Kwen Kim;Hwa Kyoung Shin;Byung Tae Choi-.Effects of electroacupuncture on the functionality of NG2-expressing cells in perilesional brain tissue of mice following ischemic stroke)[J].中国神经再生研究（英文版）,2022(07):1556-1565

perilesional,Sishencong,rehabilitative,Pusan,Nos,PNU2019

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