Houttuynia cordata is traditionally used as phytoantibiotics for treating lung disease in China.Houttuynia cordata polysaccharides(HCPs)have been reported to alleviate influenza virus-induced intestinal and lung immune injury by regulating the gut-lung axis.The present study aims to investigate the effects and mechanisms of HCPs on ulcerative colitis(UC).Male C57BL/6 mice were induced by dextran sodium sulfate(DSS)to establish the UC animal model.Our results showed that HCPs significantly reduced the weight loss and the shortening of colon length in colitis mice,and relieved the pathological damage of colon mucosa and inhibited the expression of pro-inflammatory cytokines such as TNF-a,IL-1β,IL-6,etc.It was suggested that HCPs could significantly improve DSS-induced colitis in mice.HCPs directly protected intestinal epithelial cells,ameliorated epithelial barrier dysfunction and cell ap-optosis,which was also proved in H202 stimulated cell apoptosis model.HCPs inhibited inflammation in the colon,which was related to suppressing the infiltration of macrophages,inhibiting the expression of pro-inflammatory cytokines and proteins(TLR4,NF-κB),and restoring the dysfunction of Th 17 and Treg cells.HCPs also restored the alteration of intestinal flora induced by DSS,increased the abundance of Firmicutes and Bacteroides,and reduced the abundance of Proteobacteria.This study confirmed the protective ef-fect of Houttuynia cordata polysaccharide extracted from traditional Chinese medicine on ulcerative colitis,of which the mechanism was closely related to the maintenance of intestinal homeostasis(intestinal barrier,immune cells,and intestinal bacteria).

CEN Lifeng;YI Tong;HAO Yuanzhen;SHI Chenchen;SHI Xunlong;LU Yan;CHEN Daofeng;ZHU Haiyan

Department of Biological Medicines&Shanghai Engineering Research Center of ImmunoTherapeutics,School of Pharmacy,Fudan University,Shanghai 200000,China;Division of Spine,Department of Orthopedics,Tongji Hospital,Shanghai 200000,China;Department of Natural Medicine,School of Pharmacy,Fudan University,Shanghai 200000,China

中国天然药物

[1]CEN Lifeng;YI Tong;HAO Yuanzhen;SHI Chenchen;SHI Xunlong;LU Yan;CHEN Daofeng;ZHU Haiyan-.Houttuynia cordata polysaccharides alleviate ulcerative colitis by restoring intestinal homeostasis)[J].中国天然药物,2022(12):914-924

phytoantibiotics,optosis

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