In the present study, an oligosaccharide SPO-1 from Spirulina platensis was prepared by glycosidase from a marine bacterium. The prebiotic activity of SPO-1 on the growth of Lactobacillus paracasei and Bifidobacterium animalis, and its effect on human gut microbiota were examined in vitro. The molecular weight of the tetrasaccharide SPO-1 was 650.2 Da, and it was mainly composed of glucose with α-type glycosidic linkages. The prebiotic activity score of SPO-1 was the highest for the growth of probiotic strains L. paracasei and B. animalis. Furthermore, as fermentation proceeded, SPO-1 was gradually degraded and utilized by intestinal bacteria. The results showed that after treatment with SPO-1, carbohydrate consumption and short-chain fatty acids levels were increased, especially those of i-butyric and i-valeric acids. Moreover, SPO-1 significantly promoted the abundance, diversity and composition of gut microbiota, especially stimulating the growth of Bacteroides, Escherichia-Shigella and Megamonas. In addition, the change in intestinal microbiota function predicted by phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) after treatment with SPO-1 is mainly related to the terms "carbohydrate metabolism" and "amino acid metabolism". These results suggest that SPO-1 is a potential oligosaccharide in regulation of intestinal microbiota.

Bingna Cai;Xiangxi Yi;Qian Han;Jianyu Pan;Hua Chen;Huili Sun;Peng Wan

Key Laboratory of Tropical Marine Bio-Resources and Ecology/Guangdong Key Laboratory of Marine Materia Medica,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou,510301 Guangdong,China;Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou,511458 Guangdong,China;School of Pharmacy,Guangxi University of Chinese Medicine,Nanning,530200 Guangxi,China;Innovation Academy of South China Sea Ecology and Environmental Engineering(ISEE),Chinese Academy of Sciences,Guangzhou,510000 Guangdong,China

食品科学与人类健康（英文）

[1]Bingna Cai;Xiangxi Yi;Qian Han;Jianyu Pan;Hua Chen;Huili Sun;Peng Wan-.Structural characterization of oligosaccharide from Spirulina platensis and its effect on the faecal microbiota in vitro)[J].食品科学与人类健康（英文）,2022(01):109-118

glycosidase

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