Background : Diabetes-i nduced oxidative stress can have adverse effects on sperm and its DNA integrity. The Ashrasi date palm (ADP) has potent antioxidant properties. The aim of this study was to evaluate the antioxidant effect of ADP hydroalcoholic extract on sperm parameters and sperm DNA fragmentation in diabetic rats. Methods : Forty male rats were randomly divided into five groups ( n = 7): 1, control; 2, diabetic; 3– 5, diabetic + ADP (30, 90 and 270 mg/kg for groups 3, 4 and 5, re-spectively). After preparation of ADP extract and its phytochemical screening, it was administered orally to rats, once a day for 5 weeks. At the end of the study, sperm parameters and sperm DNA fragmentation in all groups were investigated. Results : At doses of 90 and 270 mg/kg, ADP extract significantly increased the sperm viability compared to diabetic group 2 ( p = 0.04 and p = 0.03, respectively) and resulted in a significant decrease in immotile sperm ( p = 0.002 and p = 0.006, respectively). At a dose of 270 mg/kg, a considerable enhancement of forward sperm motility was observed ( p = 0.04) and there was a significant decrease in sperm DNA fragmentation ( p = 0.04). Conclusions : The findings of the present study show for the first time that the hy-droalcoholic extract of ADP has protective and antioxidant effects against diabetes- induced oxidative stress and can improve sperm parameters and protect sperm DNA integrity.

Morteza Hosseinipour;Rezvan Asgari;Javid Kermani;Nader Goodarzi;Mitra Bakhtiari

Faculty of Veterinary Medicine,Razi Universtiy,Kermanshah,Iran;Medical Biology Research Center, Health Technology Institute,Kermanshah University of Medical Sciences, Kermanshah,Iran;Department of Basic and Pathobiological Sciences,Faculty of Veterinary Medicine,Razi Universtiy,Kermanshah,Iran;Fertility and Infertility Research Center,Health Technology Institute,Kermanshah University of Medical Sciences, Kermanshah,Iran

动物模型与实验医学（英文）

[1]Morteza Hosseinipour;Rezvan Asgari;Javid Kermani;Nader Goodarzi;Mitra Bakhtiari-.The antioxidant effects of hydroalcoholic extract of Ashrasi date palm on sperm parameters and DNA fragmentation in diabetic rats)[J].动物模型与实验医学（英文）,2022(03):281-287

Ashrasi,nduced,immotile,droalcoholic

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