Our previous studies demonstrated that oral vitamin A supplementation during late-stage pregnancy and the neonatal stage enhances birth weight,growth performance,and mRNA expression related to muscle and preadipocyte development in beef cattle.The alcohol dehydrogenase 1C(ADH1C)c.-64T＞C geno-type also correlated with vitamin A concentration in beef production.This study aimed to investigate the effects of vitamin A supplementation on the muscle development and vitamin A metabolism in weaned beef calves with different ADH1C genotypes.Twenty male calves(90 d of age;initial BW:89.03 kg[SD 8.60])were stratified according to ADH1C genotype and vitamin A treatment(duration:3 months)and randomly assigned to 4 groups with a 2×2 factorial arrangement.Vitamin A treatments included the following:control(10,000 IU/kg of as-fed,a.TT type;b.TC type);treatment(40,000 IU/kg of as-fed,c.TT type;and d.TC type).Parameters including BW,FI,blood,longissimus dorsi muscle,and liver status during the experimental period were analyzed using the generalized linear model(GLM)procedure and Tukey's test by SAS 9.4 program.Serum vitamin A was significantly increased(P＜0.05)in the vitamin A treatment group at 4 and 6 months of age.TT type calves showed higher serum vitamin A concentration(P＜0.05)than the TC type calves.Serum triglyceride and non-esterified fatty acid(NEFA)levels increased(P＜0.05)in the treatment group compared with the control at 6 months of age.However,BW,ADG and Fl showed no differences between the groups.In addition,mRNA expression in longissimus dorsi muscle revealed upregulation of paired box 7(PAX7)(P＜0.05)after the vitamin A treatment period based on biopsy results.Both ADH1C and aldehyde dehydrogenase(ALDH)1A1 mRNA expression was downregulated(P＜0.01)by vitamin A supplementation.The TC type of ADH1C showed higher mRNA expression than the TT type.However,no effect was observed on adipogenic mRNA expression(pre-adipocyte factor-1[PREF-1],peroxisome proliferator-activated receptor gamma[PPARγ],fatty acid binding protein 4[FABP4])in all groups.Our findings suggest that weaned calves treated with vitamin A may promote the storage of satellite cells by elevating PAX7 gene expression in the muscle.The TC type calves may show increased capacity for vitamin A metabolism,which can be used in genetically customizing feed management to maximize beef production in the calves.

Xue Cheng Jin;Dong Qiao Peng;Seong Jin Kim;Na Yeon Kim;Jalil Ghassemi Nejad;Danil Kim;Stephen B.Smith;Hong Gu Lee

Department of Animal Science and Technology,Sanghvi College of Life Sciences,Konkuk University,Seoul 05029,Republic of Korea;Asia Pacific Ruminant Institute,Icheon 17385,Republic of Korea;Farm Animal Clinical Training and Research Center,Institute of Green-Bio Science and Technology,Seoul National University,Pyeongchang 25354,Republic of Korea;Department of Animal Science,Texas A&M University,College Station,TX 77843,USA

动物营养（英文）

[1]Xue Cheng Jin;Dong Qiao Peng;Seong Jin Kim;Na Yeon Kim;Jalil Ghassemi Nejad;Danil Kim;Stephen B.Smith;Hong Gu Lee-.Vitamin A supplementation downregulates ADH1C and ALDH1A1 mRNA expression in weaned beef calves)[J].动物营养（英文）,2022(03):372-381

calves,64T

Vitamin,supplementation,downregulates,ADH1C,ALDH1A1,expression,weaned,beef,Our,previous,studies,demonstrated,that,oral,vitamin,during,stage,pregnancy,neonatal,enhances,birth,weight,growth,performance,muscle,preadipocyte,development,cattle,alcohol,dehydrogenase,also,correlated,concentration,production,This,study,aimed,investigate,effects,metabolism,different,genotypes,Twenty,male,initial,BW,were,stratified,according,duration,months,randomly,assigned,groups,factorial,arrangement,treatments,included,following,control,IU,fed,TT,Parameters,including,FI,blood,longissimus,dorsi,liver,status,experimental,period,analyzed,using,generalized,linear,model,GLM,procedure,Tukey,test,by,SAS,program,Serum,was,significantly,increased,showed,higher,serum,than,triglyceride,esterified,fatty,acid,NEFA,levels,compared,However,ADG,Fl,differences,between,In,addition,revealed,upregulation,paired,box,PAX7,after,biopsy,results,Both,aldehyde,downregulated,observed,adipogenic,PREF,peroxisome,proliferator,activated,receptor,gamma,PPAR,binding,protein,FABP4,findings,suggest,treated,may,promote,storage,satellite,cells,elevating,capacity,which,used,genetically,customizing,feed,management,maximize

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