Background:Enteromorpha prolifera (E. prolifera) polysaccharide has become a promising feed additive with a variety of physiological activities, such as anti-oxidant, anti-cancer, anti-diabetic, immunomodulatory, hypolipidemic, and cation chelating ability. However, whether Enteromorpha polysaccharide-trace element complex supplementation regulates amino acid and fatty acid metabolism in chicken is largely unknown. This study was conducted to investigate the effects of E. prolifera polysaccharide (EP)-Zn supplementation on growth performance, amino acid, and fatty acid metabolism in chicken. Methods:A total of 184 one-day-old Ross-308 broiler chickens were randomly divided into two treatment groups with 8 replicates, 12 chickens per replicate, and fed either the basal diet (control group) or basal diet plus E. prolifera polysaccharide-Zinc (400 mg EP-Zn/kg diet). Results:Dietary EP-Zn supplementation significantly increased (P<0.05) the body weight, average daily gain, muscle antioxidant activity, serum HDL level, and reduced serum TG and LDL concentration. In addition, dietary EP-Zn supplementation could modulate ileal amino acid digestibility and upregulate the mRNA expression of amino acid transporter genes in the jejunum, ileum, breast muscle, and liver tissues (P<0.05). Compared with the control group, breast meat from chickens fed EP-Zn had higher (P<0.05) Pro and Asp content, and lower (P<0.05) Val, Phe, Gly, and Cys free amino acid content. Furthermore, EP-Zn supplementation upregulated (P<0.05) the mRNA expressions of mTOR and anti-oxidant related genes, while down-regulated protein degradation related genes in the breast muscle. Breast meat from EP-Zn supplemented group had significantly lower (P<0.05) proportions of Σn-3 PUFA, and a higher percentage ofΣn-6 PUFA and the ratio of n-6/n-3 PUFA. Besides, EP-Zn supplementation regulated lipid metabolism by inhibiting the gene expression of key enzymes involved in the fatty acid synthesis and activating genes that participated in fatty acid oxidation in the liver tissue. Conclusions:It is concluded that EP-Zn complex supplementation regulates apparent ileal amino acid digestibility, enhances amino acid metabolism, and decreases oxidative stress-associated protein breakdown, thereby improving the growth performance. Furthermore, it promotes fatty acid oxidation and restrains fat synthesis through modulating lipid metabolism-related gene expression.

Teketay Wassie;Xinyi Duan;Chunyan Xie;Ruxia Wang;Xin Wu

Key Laboratory of Agro-ecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production;Hunan Provincial Engineering Research Center for Healthy Livestock and Poultry Production,Changsha 410125, Hunan,China;College of Bioscience and Biotechnology,Hunan Agricultural University,Changsha,China;Institute of Biological Resources,Jiangxi Academy of Sciences,Nanchang 330096,China

畜牧与生物技术杂志（英文版）

[1]Teketay Wassie;Xinyi Duan;Chunyan Xie;Ruxia Wang;Xin Wu-.Dietary Enteromorpha polysaccharide-Zn supplementation regulates amino acid and fatty acid metabolism by improving the antioxidant activity in chicken)[J].畜牧与生物技术杂志（英文版）,2022(03):878-896

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