Advances in biomimetic three-dimensional(3D)melanoma models have brought new prospects of drug screening and disease modeling,since their physiological relevancy for recapitulating in vivo tumor architectures is more accurate than traditional two-dimensional(2D)cell culture.Gelatin methacryloyl(GelMA)is widely used as a tissue-engineered scaffold hydrogel for 3D cell culture.In the present study,an in vitro 3D malignant melanoma model based on GelMA was fabricated to evaluate the efficiency of hypericin(Hy)-loaded microemulsion(ME)in photodynamic therapy against melanoma.The ME was produced by the spontaneous emulsification method to enhance the bioavailability of Hy at tumor sites.Hy-loaded MEs were applied to a 3D malignant melanoma model made using 6％GelMA and the co-culture of B16F10 and Balb/c 3T3 cells,followed by crosslinking using violet light(403 nm).The observation revealed excellent cell viability and the presence of F-actin cytoskeleton network.Hy-loaded MEs exhibited higher phototoxicity and cell accumulation(about threefold)than free Hy,and the cells cultured in the 3D system displayed lower susceptibility(about 2.5-fold)than those in 2D culture.These findings indicate that the developed MEs are potential delivery carriers for Hy;furthermore,GelMA hydrogel-based modeling in poly dimethylsiloxane(PDMS)molds is a user-friendly and cost-effective in vitro platform to investigate drug penetration and provide a basis for evaluating nanocarrier efficiency for skin cancer and other skin-related diseases.

Hui L.Ma;Wanlu Li;Mian Wang;Laudemir C.Varanda;Janice R.Perussi;Y.Shrike Zhang;Emanuel Carrilho

Instituto de Química de S?o Carlos,Universidade de S?o Paulo,USP,Av.Trabalhador Sao-carlense,S?o Carlos,SP 13566-590,Brazil;Instituto Nacional de Ciência e Tecnologia de Bioanalítica,INCTBio,Campinas,SP 13083-970,Brazil;Division of Engineering in Medicine,Department of Medicine,Brigham and Women's Hospital,Harvard Medical School,Cambridge,MA 02139,USA

生物设计与制造（英文）

[1]Hui L.Ma;Wanlu Li;Mian Wang;Laudemir C.Varanda;Janice R.Perussi;Y.Shrike Zhang;Emanuel Carrilho-.In vitro 3D malignant melanoma model for the evaluation of hypericin-loaded oil-in-water microemulsion in photodynamic therapy)[J].生物设计与制造（英文）,2022(04):660-673

hypericin

In,vitro,malignant,melanoma,evaluation,loaded,oil,water,microemulsion,photodynamic,therapy,Advances,biomimetic,dimensional,models,have,brought,new,prospects,drug,screening,modeling,since,their,physiological,relevancy,recapitulating,vivo,tumor,architectures,accurate,than,traditional,2D,Gelatin,methacryloyl,GelMA,widely,used,tissue,engineered,scaffold,hydrogel,present,study,was,fabricated,evaluate,efficiency,Hy,against,produced,by,spontaneous,emulsification,method,enhance,bioavailability,sites,MEs,were,applied,made,using,B16F10,Balb,3T3,cells,followed,crosslinking,violet,light,observation,revealed,excellent,viability,presence,actin,cytoskeleton,network,exhibited,higher,phototoxicity,accumulation,about,threefold,free,cultured,system,displayed,lower,susceptibility,those,These,findings,indicate,that,developed,are,potential,delivery,carriers,furthermore,poly,dimethylsiloxane,PDMS,molds,user,friendly,cost,effective,platform,investigate,penetration,provide,basis,evaluating,nanocarrier,skin,cancer,other,related,diseases

0.580612