Objective To explore the correlation between macrophages and interleukin-10 (IL-10 in the peripheral blood of breast cancer (BC) patients and the diagnostic value of joint detection. Methods BC patients (n = 50) and healthy controls (n = 40) were prospectively recruited. The percentage of circulating cluster of differentiation 14 (CD 14) macrophage cells was analyzed by flow cytometry, and an enzyme-linked immunosorbent assay (ELISA) was used to detect IL-10 expression levels. Receiver operating characteristic (ROC) curves were used to verify the diagnostic value of the models based on the expression of CD14 macrophage cell populations and IL-10. In addition, the association between model expression and clinicopathological characteristics was investigated. Another 30 patients with BC and 30 with benign breast disease were selected to validate the IL-10 and CD14 macrophage joint detection model using the same method. Results CD14 macrophage and IL-10 expression levels in BC patients were higher than those in healthy controls (P < 0.05). The ROC curve showed that the area under the curve (AUC) of CD14+ macrophages combined with IL-10 was 0.830, the sensitivity was 72.0％, and the specificity was 87.5％. Its diagnostic efficiency was better than all other single and joint detections. Correlation analysis of clinicopathological features showed that IL-10 and CD14+ macrophage joint detection was significantly correlated with tumor size, tumor-node-metastasis (TNM) stage, and lymph node, estrogen receptor (ER), and Ki-67 expression (P < 0.05). The validation analysis results were consistent with the test results. Conclusion Peripheral blood macrophages can be an independent diagnostic marker for BC. Joint detection of CD14- macrophages and IL-10 suggests poor prognosis, which has unlimited potential to guide BC development and provides a new theory for studying tumor-associated macrophages in BC.

Mengting Dong;Jiayu Sheng;Chunyang Li;Patiguli·JIAPAER;Xiaofei Li;Minjia Yuan;Xiaohong Xue;Ke Jiang

Department of Breast Diseases,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine,Shanghai University of Traditional Chinese Medicine,Shanghai 200437,China

肿瘤学与转化医学（英文）

[1]Mengting Dong;Jiayu Sheng;Chunyang Li;Patiguli·JIAPAER;Xiaofei Li;Minjia Yuan;Xiaohong Xue;Ke Jiang-.CD14 macrophage and IL-10 levels in the peripheral blood of breast cancer patients and their diagnostic value)[J].肿瘤学与转化医学（英文）,2022(04):165-172

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