Objective:Emerging studies have demonstrated the promising clinical value of circulating tumor cells(CTCs)for diagnosis,disease assessment,treatment monitoring and prognosis in epithelial ovarian cancer.However,the clinical application of CTC remains restricted due to diverse detection techniques with variable sensitivity and specificity and a lack of common standards.Methods:We enrolled 160 patients with epithelial ovarian cancer as the experimental group,and 90 patients including 50 patients with benign ovarian tumor and 40 healthy females as the control group.We enriched CTCs with immunomagnetic beads targeting two epithelial cell surface antigens(EpCAM and MUC1),and used multiple reverse transcription-polymerase chain reaction(RT-PCR)detecting three markers(EpCAM,MUC1 and WT1)for quantification.And then we used a binary logistic regression analysis and focused on EpCAM,MUC1 and WT1 to establish an optimized CTC detection model.Results:The sensitivity and specificity of the optimized model is 79.4％and 92.2％,respectively.The specificity of the CTC detection model is significantly higher than CA125(92.2％vs.82.2％,P=0.044),and the detection rate of CTCs was higher than the positive rate of CA125(74.5％vs.58.2％,P=0.069)in early-stage patients(stage Ⅰ and Ⅱ).The detection rate of CTCs was significantly higher in patients with ascitic volume≥500 mL,suboptimal cytoreductive surgery and elevated serum CA125 level after 2 courses of chemotherapy(P＜0.05).The detection rate of CTCEpCAM+and CTCMUC1+was significantly higher in chemo-resistant patients(26.3％vs.11.9％;26.4％vs.13.4％,P＜0.05).The median progression-free survival time for CTCMUC1+patients trended to be longer than CTCMUC1-patients,and overall survival was shorter in CTCMUC1+patients(P=0.043).Conclusions:Our study presents an optimized detection model for CTCs,which consists of the expression levels of three markers(EpCAM,MUC1 and WT1).In comparison with CA125,our model has high specificity and demonstrates better diagnostic values,especially for early-stage ovarian cancer.Detection of CTCEpCAM+and CTCMUC1+had predictive value for chemotherapy resistance,and the detection of CTCMUC1+suggested poor prognosis.

Tongxia Wang;Yan Gao;Xi Wang;Junrui Tian;Yuan Li;Bo Yu;Cuiyu Huang;Hui Li;Huamao Liang;David M.Irwin;Huanran Tan;Hongyan Guo

Department of Obstetrics and Gynecology,Peking University Third Hospital,Beijing 100191,China;Department of Pharmacology,School of Basic Medical Sciences,Peking University Health Science Center,Beijing 100191,China;Department of Laboratory Medicine and Pathobiology,University of Toronto,Toronto M5S1A8,Canada

中国癌症研究（英文版）

[1]Tongxia Wang;Yan Gao;Xi Wang;Junrui Tian;Yuan Li;Bo Yu;Cuiyu Huang;Hui Li;Huamao Liang;David M.Irwin;Huanran Tan;Hongyan Guo-.Establishment of an optimized CTC detection model consisting of EpCAM,MUC1 and WT1 in epithelial ovarian cancer and its correlation with clinical characteristics)[J].中国癌症研究（英文版）,2022(02):95-108

immunomagnetic,ascitic,CTCEpCAM+and,CTCMUC1+was,CTCMUC1+patients,CTCMUC1,CTCMUC1+had,CTCMUC1+suggested

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