典型文献
Rapid fabrication of zwitterionic sulfobetaine vinylimidazole-based monoliths via photoinitiated copolymerization for hydrophilic interaction chromatography
文献摘要：
Zwitterionic sulfobetaine-based monolithic stationary phases have attracted increasing attention for their use in hydrophilic interaction chromatography.In this study,a novel hydrophilic polymeric monolith was fabricated through photo-initiated copolymerization of 3-(3-vinyl-1-imidazolio)-l-propanesulfonate(SBVI)with pentaerythritol triacrylate using methanol and tetrahydrofuran as the porogenic system.Notably,the duration for the preparation of this novel monolith was as little as 5 min,which was significantly shorter than that required for previously reported sulfobetaine-based monoliths prepared via conventional thermally initiated copolymerization.Moreover,these monoliths showed good morphology,permeability,porosity(62.4％),mechanical strength(over 15 MPa),column efficiency(51,230 plates/m),and reproducibility(relative standard deviations for all analytes were lower than 4.6％).Mechanistic studies indicated that strong hydrophilic and negative electrostatic interactions might be responsible for the retention of polar analytes on the zwitterionic SBVI-based monolith.In particular,the resulting monolith exhibited good anti-protein adhesion ability and low nonspecific protein adsorption.These excellent features seem to favor its application in bioanalysis.Therefore,the novel zwitterionic sulfobetaine-based monolith was successfully employed for the highly selective separation of small bioactive compounds and the efficient enrichment of N-glycopeptides from complex samples.In this study,we prepared a novel zwitterionic sulfobetaine-based monolith with good performance and developed a simpler and faster method for preparation of zwitterionic monoliths.
文献关键词：
中图分类号：
作者姓名：
Qiqin Wang;Lingjue Sun;Huihui Wu;Ning Deng;Xianglong Zhao;Jingwei Zhou;Tingting Zhang;Hai Han;Zhengjin Jiang
作者机构：
Institute of Pharmaceutical Analysis,College of Pharmacy,Jinan University,Guangzhou,510632,China;Anhui Prevention and Treatment Center for Occupational Disease,Anhui No.2 Provincial People's Hospital,Hefei,230041,China;Key Laboratory of Environment-Friendly Chemistry and Application of Ministry of Education,Lab of Biochemistry,College of Chemistry,Xiangtan University,Xiangtan,Hunan,411105,China;Department of Pharmacy and Guangdong Province Key Laboratory of Pharmacodynamic Constituents of Traditional Chinese Medicine&New Drug Research,Jinan University,Guangzhou,510632,China
文献出处：
引用格式：
[1]Qiqin Wang;Lingjue Sun;Huihui Wu;Ning Deng;Xianglong Zhao;Jingwei Zhou;Tingting Zhang;Hai Han;Zhengjin Jiang-.Rapid fabrication of zwitterionic sulfobetaine vinylimidazole-based monoliths via photoinitiated copolymerization for hydrophilic interaction chromatography)[J].药物分析学报（英文）,2022(05):783-790
A类：
vinylimidazole,photoinitiated,imidazolio,SBVI,pentaerythritol,porogenic
B类：
Rapid,fabrication,zwitterionic,sulfobetaine,monoliths,copolymerization,hydrophilic,chromatography,Zwitterionic,monolithic,stationary,phases,have,attracted,increasing,attention,their,use,In,this,study,novel,polymeric,was,fabricated,through,propanesulfonate,triacrylate,using,methanol,tetrahydrofuran,system,Notably,duration,preparation,little,which,significantly,shorter,that,required,previously,reported,prepared,conventional,thermally,Moreover,these,showed,good,morphology,permeability,porosity,mechanical,strength,column,efficiency,plates,reproducibility,relative,standard,deviations,analytes,were,lower,Mechanistic,studies,indicated,strong,negative,electrostatic,interactions,might,responsible,retention,polar,particular,resulting,exhibited,anti,protein,adhesion,nonspecific,adsorption,These,excellent,features,seem,favor,its,application,bioanalysis,Therefore,successfully,employed,highly,selective,separation,small,bioactive,compounds,efficient,enrichment,glycopeptides,from,complex,samples,performance,developed,simpler,faster,method
AB值：
0.52744
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