Objective: To analyze the role of miRNA-27a in regulating the immune function of the pulmonary tuberculosis rat model. Methods:40 healthy female rats were divided into 4 groups: healthy control group, model group, silence group, and overexpression group, with 10 rats in each group. The control group was not treated , and the other three groups of rats were injected with 200 μL of Mycobacterium bacilli suspension through the tail vein to establish a rat model of pulmonary tuberculosis, in which the model group was not treated, and the silence group was injected with 10 μL of miRNA-27 inhibition vector lentiviral suspension, and the overexpression group was injected with 10μL of miRNA-27 expression vector lentiviral suspension. The expression level of miRNA-27a, peripheral blood T lymphocyte subsets, thymus index, inflammatory index, and TLR4/NF-κB signal pathway-related protein level in the four groups of rats were compared and analyzed. Results: Compared with the healthy control group, the expression levels of the silence group and model group were significantly decreased, while the expression level of the overexpression group was significantly increased; Compared with the model group and addiction group, the expression level of the overexpression group was significantly increased, with significant differences( P<0.05). Compared with the healthy control group, CD3+, CD4+, CD4+/CD8+, thymus index, IL-10, and IκB in the model group, silence group, and overexpression group were significantly higher than those in the control group;The levels of CD3+, CD4+, CD4+/CD8+, thymus index, IL-10, IκB decreased significantly, with significant differences ( P<0.05); Compared with the overexpression group, CD8+, IL-6, TNF-α, TLR4, NF-κBp65, and p-IκB levels were significantly lower; The levels of CD3+, CD4+, CD4+/CD8+, thymic index, IL-10, and IκB levels were significantly increased, with significant differences ( P<0.05). Compared with the silence group, the levels of CD3+, CD4+, CD4+/CD8+, thymic index, IL-10, and IκB were significantly increased and the levels of CD8+, IL-6, TNF-α, TLR4, NF-κBp65, and p-IκB were significantly lower in the overexpression group, with significant differences (P<0.05). Conclusion:Overexpression of miRNA-27a can inhibit the TLR4/NF-κB signal pathway and regulate the immune function of rats with pulmonary tuberculosis, which in turn reduce lung injury in rats.

XU Bai-lan

Department of Respiratory and Critical Care Medicine,the First Hospital of Quanzhou,Quanzhou Fujian 362000,China

中国生物医学工程学报（英文版）

[1]XU Bai-lan-.Role of miRNA-27a in Regulating the Immune Function of Pulmonary Tuberculosis Rat Model)[J].中国生物医学工程学报（英文版）,2022(03):112-119,138

Role,miRNA,27a,Regulating,Immune,Function,Pulmonary,Tuberculosis,Rat,Model,Objective,To,role,regulating,immune,function,pulmonary,tuberculosis,model,Methods,healthy,female,rats,were,divided,into,groups,control,silence,overexpression,each,was,not,treated,other,three,injected,Mycobacterium,bacilli,suspension,through,tail,vein,establish,which,inhibition,vector,lentiviral,peripheral,blood,lymphocyte,subsets,thymus,inflammatory,TLR4,signal,pathway,related,protein,four,compared,analyzed,Results,Compared,levels,significantly,decreased,while,increased,addiction,differences,CD3+,CD4+,CD8+,higher,than,those,Bp65,lower,thymic,Conclusion,Overexpression,regulate,turn,reduce,lung,injury

0.313267