Objective:To determine whether Schisandrin B(Sch B)attenuates early brain injury(EBI)in rats with subarachnoid hemorrhage(SAH).Methods:Sprague-Dawley rats were divided into sham(sham operation),SAH,SAH+vehicle,and SAH+Sch B groups using a random number table.Rats underwent SAH by endovascular perforation and received Sch B(100 mg/kg)or normal saline after 2 and 12 h of SAH.SAH grading,neurological scores,brain water content,Evan's blue extravasation,and terminal transferase-mediated dUTP nick end-labeling(TUNEL)staining were carried out 24 h after SAH.Immunofluorescent staining was performed to detect the expressions of ionized calcium binding adapter molecule 1(Iba-1)and myeloperoxidase(MPO)in the rat brain,while the expressions of B-cell lymphoma 2(Bcl-2),Bax,Caspase-3,nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing 3(NLRP3),apoptosis-associated speck-like protein containing the caspase-1 activator domain(ASC),Caspase-1,interleukin(IL)-1 β,and IL-18 in the rat brains were detected by Western blot.Results:Compared with the SAH group,Sch B significantly improved the neurological function,reduced brain water content,Evan's blue content,and apoptotic cells number in the brain of rats(P＜0.05 or P＜0.01).Moreover,Sch B decreased SAH-induced expressions of Iba-1 and MPO(P＜0.01).SAH caused the elevated expressions of Bax,Caspase-3,NLRP3,ASC,Caspase-1,IL-1 β,and IL-18 in the rat brain(P＜0.01),all of which were inhibited by Sch B(P＜0.01).In addition,Sch B increased the Bcl-2 expression(P＜0.01).Conclusion:Sch B attenuated SAH-induced EBI,which might be associated with the inhibition of neuroinflammation,neuronal apoptosis,and the NLRP3 inflammatory signaling pathway.

CHEN Song;DING Yi-hang;SHI Song-sheng;TU Xian-kun

Department of Neurosurgery,Fujian Medical University Union Hospital,Neurosurgery Research Institute of Fujian Province,Fuzhou,350001,China

中国结合医学杂志（英文版）

[1]CHEN Song;DING Yi-hang;SHI Song-sheng;TU Xian-kun-.Schisandrin B Inhibits NLRP3 Inflammasome Pathway and Attenuates Early Brain Injury in Rats of Subarachnoid Hemorrhage)[J].中国结合医学杂志（英文版）,2022(07):594-602

Inflammasome,SAH+vehicle,SAH+Sch,Immunofluorescent

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