Objective:To investigate whether Lingbao Huxin Pill(LBHX)protects against acute myocardial infarction(AMI)at the infarct border zone(IBZ)of myocardial tissue by regulating apoptosis and inflammation through the sirtuin 1(SIRT1)-mediated forkhead box protein O1(FOXO1)and nuclear factor-κ B(NF-κB)signaling pathways.Methods:Six-week-old Wistar rats with normal diet were randomized into the sham,the model,Betaloc(0.9 mg/kg daily),LBHX-L(0.45 mg/kg daily),LBHX-M(0.9 mg/kg daily),LBHX-H(1.8 mg/kg daily),and LBHX+EX527(0.9 mg/kg daily)groups according to the method of random number table,13 in each group.In this study,left anterior descending coronary artery(LADCA)ligation was performed to induce an AMI model in rats.The myocardial infarction area was examined using a 2,3,5-triphenyttetrazolium chloride solution staining assay.A TdT-mediated dUTP nick-end labeling(TUNEL)assay was conducted to assess cardiomyocyte apoptosis in the IBZ.The histopathology of myocardial tissue at the IBZ was assessed with Heidenhain,Masson and hematoxylin-eosin(HE)staining assays.The expression levels of tumor necrosis factor α(TNF-α),interleukin(IL)-6,IL-1 β,and intercellular adhesion molecule-1 were measured using enzyme-linked immunosorbent assays(ELISAs).The mRNA expressions of SIRT1 and FOXO1 were detected by real-time qPCR(RT-qPCR).The protein expressions of SIRT1,FOXO1,SOD2,BAX and NF-κ B p65 were detected by Western blot analysis.Results:The ligation of the LADCA successfully induced an AMI model.The LBHX pretreatment reduced the infarct size in the AMI rats(P＜0.01).The TUNEL assay revealed that LBHX inhibited cardiomyocyte apoptosis at the IBZ.Further,the histological examination showed that the LBHX pretreatment decreased the ischemic area of myocardial tissue(P＜0.05),myocardial interstitial collagen deposition(P＜0.05)and inflammation at the IBZ.The ELISA results indicated that LBHX decreased the serum levels of inflammatory cytokines in the AMI rats(P＜0.05 or P＜0.01).Furthermore,Western blot analysis revealed that the LBHX pretreatment upregulated the protein levels of SIRT1,FOXO1 and SOD2(P＜0.05)and downregulated NF-κ B p65 and BAX expressions(P＜0.05).The RT-qPCR results showed that LBHX increased the SIRT1 mRNA and FOXO1 mRNA levels(P＜0.05).These protective effects,including inhibiting apoptosis and alleviating inflammation in the IBZ,were partially abolished by EX527,an inhibitor of SIRT1.Conclusion:LBHX could protect against AMI by suppressing apoptosis and inflammation in AMI rats and the SIRT1-mediated FOXO1 and NF-κ B signaling pathways were involved in the cardioprotection effect of LBHX.

TAN Yu;BIE Yu-long;CHEN Li;ZHAO Yi-han;SONG Lei;MIAO Li-na;YU Yan-qiao;CHAI Hua;MA Xiao-juan;SHI Da-zhuo

National Clinical Research Center for Chinese Medicine Cardiology,Xiyuan Hospital,China Academy of Chinese Medical Sciences,Beijing(100091),China;Peking University Traditional Chinese Medicine Clinical Medical School(Xiyuan Hospital),Beijing(100191),China;Department of Graduate School,Beijing University of Chinese Medicine,Beijing(100029),China

中国结合医学杂志（英文版）

[1]TAN Yu;BIE Yu-long;CHEN Li;ZHAO Yi-han;SONG Lei;MIAO Li-na;YU Yan-qiao;CHAI Hua;MA Xiao-juan;SHI Da-zhuo-.Lingbao Huxin Pill Alleviates Apoptosis and Inflammation at Infarct Border Zone through SIRT1-Mediated FOXO1 and NF-κ B Pathways in Rat Model of Acute Myocardial Infarction)[J].中国结合医学杂志（英文版）,2022(04):330-338

Lingbao,Huxin,LBHX,IBZ,Betaloc,LBHX+EX527,triphenyttetrazolium

Pill,Alleviates,Apoptosis,Inflammation,Border,Zone,through,SIRT1,Mediated,FOXO1,Pathways,Rat,Model,Acute,Myocardial,Infarction,Objective,To,investigate,whether,protects,against,acute,myocardial,infarction,AMI,border,zone,tissue,by,regulating,apoptosis,inflammation,sirtuin,mediated,forkhead,box,protein,nuclear,signaling,pathways,Methods,Six,week,old,Wistar,rats,normal,diet,were,randomized,into,sham,model,daily,groups,according,method,number,table,each,this,study,left,anterior,descending,coronary,artery,LADCA,ligation,was,performed,area,examined,using,chloride,solution,staining,TdT,dUTP,nick,labeling,TUNEL,conducted,cardiomyocyte,histopathology,assessed,Heidenhain,Masson,hematoxylin,eosin,HE,assays,levels,tumor,necrosis,interleukin,intercellular,adhesion,molecule,measured,enzyme,linked,immunosorbent,ELISAs,expressions,detected,real,qPCR,SOD2,BAX,p65,blot,analysis,Results,successfully,induced,pretreatment,reduced,size,revealed,that,inhibited,histological,examination,showed,decreased,ischemic,interstitial,collagen,deposition,results,indicated,serum,inflammatory,cytokines,Furthermore,upregulated,downregulated,increased,These,protective,effects,including,inhibiting,alleviating,partially,abolished,inhibitor,Conclusion,could,suppressing,involved,cardioprotection

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