Objective:To investigate the effects of Shenfu Injection(SFI)on endothelial damage in a porcine model of hemorrhagic shock(HS).Methods:After being bled to a mean arterial pressure of 40±3 mm Hg and held for 60 min,32 pigs were treated with a venous injection of either shed blood(transfusion group),shed blood and saline(saline group),shed blood and SFI(SFI group)or without resuscitation(sham group).Venous blood samples were collected and analyzed at baseline and 0,1,2,4,and 6 h after HS.Tumor necrosis factor-α(TNF-α),serum interleuking(IL)-6,and IL-10 levels were measured by enzyme-linked immunosorbent assay(ELISA);expressions of vascular cell adhesion molecule-1(VCAM-1),intercellular adhesion molecule 1(ICAM-1),von Willebrand factor(vWF),plasminogen activator inhibitor-1(PAI-1)and Bcl-2,Bax,and caspase-3 proteins were determined by Western blot.Results:The serum level of TNF-α in the SFI group was significantly lower than in the other groups at 0,1,and 2 h after HS,while the level of IL-6 was lower at 4 and 6 h compared with the saline group(P＜0.01 or P＜0.05).The concentration of serum IL-10 was significantly higher in the SFI group than in the other groups at 0,1,4,and 6 h after HS(P＜0.01).Western blot and immunohistochemistry of vascular tissue showed that the expression of caspase-3 was downregulated,and that of Bcl-2 and Bax was upregulated in the SFI group compared to other groups(P＜0.05).Conclusion:SFI attenuated endothelial injury in the porcine model of HS by inhibiting cell apoptosis,suppressing the formation of proinflammatory cytokines,and reducing endothelial activation.

ZHANG Ming-qing;ZHANG Qiang;YUAN Wei;WU Jun-yuan;LIANG Yong;QIN Hong-jie;LI Chun-sheng

Department of Emergency Medicine,Beijing Friendship Hospital,Capital Medical University,Beijing(100050),China;Department of Critical Care Medicine,Peking University Third Hospital,Beijing(100191),China;Department of Emergency Medicine,Beijing Chaoyang Hospital,Capital Medical University,Beijing(100020),China;Department of Emergency Medicine,Beijing Luhe Hospital,Capital Medical University,Beijing(101199),China

中国结合医学杂志（英文版）

[1]ZHANG Ming-qing;ZHANG Qiang;YUAN Wei;WU Jun-yuan;LIANG Yong;QIN Hong-jie;LI Chun-sheng-.Protective Effect of Shenfu Injection on Vascular Endothelial Damage in a Porcine Model of Hemorrhagic Shock)[J].中国结合医学杂志（英文版）,2022(09):794-801

Shenfu,interleuking

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