Objective This study was designed to provide the evidences on the toxicokinetics of microplastics(MPs) and nanoplastics (NPs) in the bodies of mammals. Methods 100 nm, 3 μm, and 10 μm fluorescent polystyrene (PS) beads were administered to mice once by gavage at a dose of 200 mg/kg body weight. The levels and change of fluorescence intensity in samples of blood, subcutaneous fat, perirenal fat, peritesticular fat, cerebrum, cerebellum, testis, and epididymis were measured at 0.5, 1, 2, and 4 h after administration using an IVIS Spectrum small-animal imaging system. Histological examination, confocal laser scanning, and transmission electron microscope were performed to corroborate the findings. Results After confirming fluorescent dye leaching and impact of pH value, increased levels of fluorescence intensity in blood, all adipose tissues examined, cerebrum, cerebellum, and testis were measured in the 100 nm group, but not in the 3 and 10 μm groups except in the cerebellum and testis at 4 h for the 3 μm PS beads. The presence of PS beads was further corroborated. Conclusion After a single oral exposure, NPs are absorbed rapidly in the blood, accumulate in adipose tissues, and penetrate the blood-brain/testis barriers. As expected, the toxicokinetics of MPs is significantly size-dependent in mammals.

YANG Zuo Sen;BAI Ying Long;JIN Cui Hong;NA Jun;ZHANG Rui;GAO Yuan;PAN Guo Wei;YAN Ling Jun;SUN Wei

Institute of Preventive Medicine,China Medical University,Shenyang 110122,Liaoning,China;Institute of Chronic Diseases,Liaoning Provincial Center for Disease Control and Prevention,Shenyang 110000,Liaoning,China;Department of Maternal and Child Health,School of Public Health,China Medical University,Shenyang 110122,Liaoning,China;Department of Toxicology,School of Public Health,China Medical University,Shenyang 110122,Liaoning,China;Research Center for Universal Health,School of Public Health,China Medical University,Shenyang 110122,Liaoning,China

生物医学与环境科学（英文版）

[1]YANG Zuo Sen;BAI Ying Long;JIN Cui Hong;NA Jun;ZHANG Rui;GAO Yuan;PAN Guo Wei;YAN Ling Jun;SUN Wei-.Evidence on Invasion of Blood, Adipose Tissues, Nervous System and Reproductive System of Mice After a Single Oral Exposure: Nanoplastics versus Microplastics)[J].生物医学与环境科学（英文版）,2022(11):1025-1037

Nanoplastics,peritesticular,cerebrum

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