Objective:To investigate the effect of electro-acupuncture (EA) on vasomotor symptoms in rats with acute cerebral infarction,by observing the changes in the expression of factors related to the phosphatidylinositol (PI)system.Methods:Forty-two Wistar rats were randomly divided into 3 groups by a random number table:the control group (n=6),the model group (n=18) and the EA group (n=18).The EA group was given EA treatment at Shuigou(GV 26) instantly after modeling with middle cerebral artery occlusion (MCAO) method,while the model and control groups were not given any treatment.The degrees of neurological deficiency were evaluated using neurological severity scores (NSS) and the brain blood flow was evaluated by a laser scanning confocal microscope.Western blot analysis was conducted to detect the expression levels of G-protein subtype (Gq) and calmodulin (CaM).Competition for protein binding was conducted to detect the expression level of inositol triphosphate (IP3).Thin layer quantitative analysis was conducted to detect the expression level of diacylglycerol (DAG).The expression level of intracellular concentration of free calcium ion ([Ca2+]i) was detected by flow cytometry.Results:The NSS of the model group was significantly higher than the control group at 3 and 6 h after MCAO (P＜0.01),while the EA group was significantly lower than the model group at 6 h (P＜0.01).The cerebral blood flow in the model group was significantly lower than the control group at 1,3 and 6 h after MCAO (P＜0.01),while for the EA group it was remarkably higher than the model group at the same time points (P＜0.01).The expressions of Gq,CaM,IP3,DAG and[Ca2+]i in the model group were significantly higher than the control group (P＜0.05 or P＜0.01),and those in the EA group were significantly lower than the model group at the same time points (P＜0.05 or P＜0.01).Conclusion:EA treatment at GV 26 can effectively decrease the over-expression of related factors of PI system in rats with acute cerebral infarction,improve cerebral autonomy movement,and alleviate cerebral vascular spasm.

LI Jing;HE Ying;DU Yuan-hao;ZHANG Min;Rainer Georgi;Bernhard Kolberg;SUN Dong-wei;MA Kun;LI Yong-feng;ZHANG Xue-zhu

Key Laboratory of Acupuncture of Tianjin,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,National Clinical Research Center for Chinese Medicine Acupuncture and Moxibustion,Tianjin 300381 ,China;Department of Acupuncture and Moxibustion,First Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300381 ,China;Department of Chemical Biology,Max Planck Institute for Medical Research,Heidelberg 69120 ,Germany;Department of Internal Medicine,Mannheim Medical School of Heidelberg University,Mannheim 68167 ,Germany;Department of Chinese Medicine Rehabilitation,Shenzhen Baoan District Hospital of Traditional Chinese Medicine,Shenzhen,Guangdong Province 518000 ,China;Department of Preventive Treatment of Disease,Binhai New Area Hospital of Traditional Chinese Medicine,Fourth Teaching Hospital of Tianjin University of Traditional Chinese Medicine,Tianjin 300193 ,China;Institute of Acupuncture and Moxibustion,Shaanxi University of Chinese Medicine,Xi'an 712046 ,China

中国结合医学杂志（英文版）

[1]LI Jing;HE Ying;DU Yuan-hao;ZHANG Min;Rainer Georgi;Bernhard Kolberg;SUN Dong-wei;MA Kun;LI Yong-feng;ZHANG Xue-zhu-.Effect of Electro-acupuncture on Vasomotor Symptoms in Rats with Acute Cerebral Infarction Based on Phosphatidylinositol System)[J].中国结合医学杂志（英文版）,2022(02):145-152

Vasomotor,vasomotor,Shuigou

Effect,Electro,acupuncture,Symptoms,Rats,Acute,Cerebral,Infarction,Based,Phosphatidylinositol,System,Objective,To,investigate,electro,EA,symptoms,rats,acute,cerebral,infarction,by,observing,changes,factors,related,phosphatidylinositol,system,Methods,Forty,two,Wistar,were,randomly,divided,into,groups,number,table,control,was,given,treatment,GV,instantly,after,modeling,middle,artery,occlusion,MCAO,method,while,not,any,degrees,neurological,deficiency,evaluated,using,severity,scores,NSS,brain,blood,flow,laser,scanning,confocal,microscope,blot,analysis,conducted,levels,protein,subtype,Gq,calmodulin,CaM,Competition,binding,triphosphate,IP3,Thin,layer,quantitative,diacylglycerol,DAG,intracellular,concentration,free,calcium,Ca2+,detected,cytometry,Results,significantly,higher,than,lower,remarkably,same,points,expressions,those,Conclusion,effectively,decrease,over,improve,autonomy,movement,alleviate,vascular,spasm

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