Objective We investigated changes in the intestinal flora of children with Mycoplasma pneumoniae pneumonia (MPP). Methods Between September 2019 and November 2019, stool samples from 14 children with MPP from The Fourth Hospital of Baotou city, Inner Mongolia Autonomous Region, were collected and divided into general treatment (AF) and probiotic (AFY) groups, according to the treatment of"combined Bifidobacterium, Lactobacillus, Enterococcus, and Bacillus cereus tablets live". High-throughput 16S rDNA sequencing was used to identify intestinal flora. Results Intestinal flora abundance and diversity in children with MPP were decreased. Both Shannon and Simpson indices were lower in the AF group when compared with healthy controls (P < 0.05). When compared with healthy controls, the proportion of Enterorhabdus was lower in the AF group, while the proportion of Lachnoclostridium was higher (P < 0.05). The proportion of Bifidobacteria and Akkermansia was lower in the AFY group but Enterococcus, Lachnoclostridium, Roseburia, and Erysipelatoclostridium proportions were higher. The proportion of Escherichia coli–Shigella in the AFY group after treatment was decreased (P < 0.05). Conclusions The intestinal flora of children with MPP is disturbed, manifested as decreased abundance and diversity, and decreased Bifidobacteria. Our probiotic mixture partly improved intestinal flora disorders.

SHI Da Wei;WANG Dong Mei;NING Li Hua;LI Jing;DONG Yan;ZHANG Zhi Kun;DOU Hai Wei;WAN Rui Jie;JIA Chun Mei;XIN De LI

Department of Pediatrics,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China;Department of Pediatrics,Baotou Fourth Hospital(Baotou Children's Hospital),Baotou 014030,Inner Mongolia Autonomous Region,China;Department of Pediatrics,Beijing Chang Ping Hospital of Integrated Chinese and Western Medicine,Beijing 100096,China;Tropical Medicine Research Institute,Beijing Friendship Hospital,Capital Medical University,Beijing 100050,China

生物医学与环境科学（英文版）

[1]SHI Da Wei;WANG Dong Mei;NING Li Hua;LI Jing;DONG Yan;ZHANG Zhi Kun;DOU Hai Wei;WAN Rui Jie;JIA Chun Mei;XIN De LI-.Using 16S rDNA Sequencing Technology to Preliminarily Analyze Intestinal Flora in Children with Mycoplasma pneumoniae Pneumonia)[J].生物医学与环境科学（英文版）,2022(06):528-537

Preliminarily,AFY

Using,16S,rDNA,Sequencing,Technology,Analyze,Intestinal,Flora,Children,Mycoplasma,pneumoniae,Pneumonia,Objective,We,investigated,changes,intestinal,flora,children,MPP,Methods,Between,September,November,stool,samples,from,Fourth,Hospital,Baotou,city,Inner,Mongolia,Autonomous,Region,were,collected,divided,into,general,treatment,probiotic,groups,according,combined,Bifidobacterium,Lactobacillus,Enterococcus,Bacillus,cereus,tablets,live,High,throughput,sequencing,was,used,identify,Results,abundance,diversity,decreased,Both,Shannon,Simpson,indices,lower,when,compared,healthy,controls,When,Enterorhabdus,while,Lachnoclostridium,higher,Bifidobacteria,Akkermansia,but,Roseburia,Erysipelatoclostridium,proportions,Escherichia,coli,Shigella,after,Conclusions,disturbed,manifested,Our,mixture,partly,improved,disorders

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