Objective:To explore whether acupuncture combined with moxibustion could inhibit epithelial-mesenchymal transition in Crohn's disease by affecting the transforming growth factor β1(TGF-β1)/Smad3/Snail pathway.Methods:Sixty-three patients with Crohn's disease were randomly divided into an observation group(31 cases)receiving moxibustion at 43℃combined with acupuncture,and a control group(32 cases)receiving moxibustion at 37℃combined with sham acupuncture using a random number table.Patients were treated for 12 weeks.Crohn's Disease Activity Index(CDAI)was used to evaluate disease activity.Hematoxylin-eosin staining and transmission electron microscopy were utilized to observe the morphological and ultrastructural changes.Immunohistochemistry was used to detect the expression of transforming growth factor β1(TGF-β1),T βR1,T βR2,Smad3,Snail,E-cadherin and fibronectin in intestinal mucosal tissues.Results:The decrease of the CDAI score,morphological and ultrastructural changes were more significant in observation group.The expression levels of TGF-β1,T βR2,Smad3,and Snail in the observation group were significantly lower than those before the treatment(P＜0.05 or P＜0.01).After treatment,the expression levels of TGF-β1,T βR2,and Snail in the observation group were significantly lower than those in the control group(all P＜0.05);compared with the control group,the expression of fibronectin in the observation group was significantly decreased,and the expression of E-cadherin was significantly increased(all P＜0.05).Conclusions:Moxibustion at 43℃combined with acupuncture may suppress TGF-β1/Smad3/Snail pathway-mediated epithelial-mesenchymal transition of intestinal epithelial cells in Crohn's disease patients by inhibiting the expression levels of TGF-β 1,TβR2,Smad3,and Snail.(Registration No.ChiCTR-IIR-16007751)

GUO Sen;ZHOU Jing;ZHANG Liang;BAO Chun-hui;ZHAO Ji-meng;GAO Yan-ling;WU Pin;WENG Zhi-jun;SHI Yin

Graduate School,Shanghai University of Traditional Chinese Medicine,Shanghai(201203),China;Department of Acupuncture and Moxibustion,Shanghai Traditional Chinese Medicine Integrated Hospital,Shanghai University of Traditional Chinese Medicine,Shanghai(200082),China;Department of Acupuncture and Moxibustion,Yueyang Hospital of Integrated Traditional Chinese and Western Medicine Affiliated to Shanghai University of Traditional Chinese Medicine,Shanghai(200437),China;Key Laboratory of Acupuncture and Immunological Effects,Shanghai Research Institute of Acupuncture and Meridian,Shanghai(200030),China;Outpatient Department,Shanghai Research Institute of Acupuncture and Meridian,Shanghai(200030),China

中国结合医学杂志（英文版）

[1]GUO Sen;ZHOU Jing;ZHANG Liang;BAO Chun-hui;ZHAO Ji-meng;GAO Yan-ling;WU Pin;WENG Zhi-jun;SHI Yin-.Acupuncture and Moxibustion Inhibited Intestinal Epithelial-Mesenchymal Transition in Patients with Crohn's Disease Induced by TGF-β1/Smad3/Snail Pathway:A Clinical Trial Study)[J].中国结合医学杂志（英文版）,2022(09):823-832

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