Effective culture and management of adult tri-spine horseshoe crab,Tachypleus tridentatus can ensure that stock en-hancement programs and aquaculture systems are maintained.To explore suitable feed for animals during the breeding season,Pa-cific oyster(Ostrea gigas)(oyster group;OG)and frozen sharpbelly fish(Hemiculter leucisculus)(frozen fish group;FG)were se-lected to feed 20 T.tridentatus male and female pairs,respectively.At the end of the experiment,intestinal samples were obtained to measure digestive enzymes activities.The intestinal flora were determined by 16S rDNA sequencing.No eggs were observed in the FG and one T.tridentatus adult died.No animals died in the OG,and 9.7×104 eggs were obtained.These results show that oysters are more suitable for the development and reproduction of adult T.tridentatus than frozen fish.Additionally,the digestive enzyme activity analysis revealed that animals in the OG exhibited higher protein digestibility than those in the FG,but no significant differ-ences in lipid and carbohydrate uptake were observed between the groups.Furthermore,the intestinal flora analysis showed that op-erational taxonomic units(OTUs)and the Chao 1 index were significantly higher in the OG than in the FG,but no significant differ-ence was observed in the Shannon or Simpson indices between the groups.Our data indicate that the oyster diet improved the intes-tinal microbial diversity of T.tridentatus.We hypothesize that nutrients,such as oyster-based taurine,proteins,and highly unsatu-rated fatty acids,improve protease activity in the T tridentatus digestive tract,alter the intestinal floral structure,and improve the reproductive performance of T.tridentatus.

YING Ziwei;BAO Yuyuan;LI Yinkang;YE Guoling;ZHANG Shuhuan;XU Peng;ZHU Junhua;XIE Xiaoyong

Guangdong Provincial Key Laboratory of Fishery Ecology and Environment;South China Sea Fisheries Research Institute,Chinese Academy of Fisheries Sciences,Guangzhou 510300,China;College of Fisheries Science and Life Science of Shanghai Ocean University,Shanghai 201306,China;Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou),Guangzhou 511458,China;Guangdong Ocean Planning Research Center,Guangzhou 510322,China;Sturgeon Healthy Breeding and Medicinal Research Center,Guizhou University of Traditional Chinese Medicine,Guiyang 550025,China

中国海洋大学学报（自然科学英文版）

[1]YING Ziwei;BAO Yuyuan;LI Yinkang;YE Guoling;ZHANG Shuhuan;XU Peng;ZHU Junhua;XIE Xiaoyong-.Impact of Different Diets on Adult Tri-Spine Horseshoe Crab,Tachypleus tridentatus)[J].中国海洋大学学报（自然科学英文版）,2022(03):541-548

Diets,Horseshoe,sharpbelly,erational

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