典型文献
An optimized protocol for detecting guard cell specific gene expression by in situ RT-PCR in Brassica rapa
文献摘要：
It is important to detect specific genes expressed in the guard cells, which control gas exchange and play key roles in response to drought and salt stresses. Due to the genetic transformation of Chinese cabbage (Brassica rapa) has not been well developed, in situ RT-PCR is a valuable option for detecting guard cell specific genes. We reported an optimized protocol of in situ RT-PCR by using an FAMA homologous gene Bra001929 in Brassica rapa. FAMA in Arabidopsis has been verified to be specially expressed in guard cells. We designed specific RT-PCR primers and optimized the protocol in terms of the (a) reverse transcription time, (b) blocking time, (c) antigen-antibody incubation time, and (d) washing temperature. Our approach provides a sensitive and effective in situ RT-PCR method for locating expression in the guard cells in Brassica rapa. Moreover, we proved the guard cell specific expression of Bra001929 in the epidermis indicating its' applicability as a marker gene for guard cells of Brassica rapa.
文献关键词：
中图分类号：
作者姓名：
Yingying Song;Xinlei Guo;Jian Wu;Jianli Liang;Runmao Lin;Zifu Yan;Xiaowu Wang
作者机构：
College of Horticulture,Henan Agricultural University,Zhengzhou 450002,China;Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China
文献出处：
引用格式：
[1]Yingying Song;Xinlei Guo;Jian Wu;Jianli Liang;Runmao Lin;Zifu Yan;Xiaowu Wang-.An optimized protocol for detecting guard cell specific gene expression by in situ RT-PCR in Brassica rapa)[J].园艺学报（英文版）,2022(03):311-318
A类：
Bra001929
B类：
An,optimized,protocol,detecting,guard,specific,expression,by,situ,Brassica,rapa,It,important,genes,expressed,cells,which,control,gas,exchange,play,key,roles,response,drought,salt,stresses,Due,genetic,transformation,Chinese,cabbage,has,not,been,well,developed,valuable,option,We,reported,using,FAMA,homologous,Arabidopsis,verified,specially,designed,primers,terms,reverse,transcription,blocking,antigen,antibody,incubation,washing,temperature,Our,approach,provides,sensitive,effective,method,locating,Moreover,proved,epidermis,indicating,its,applicability,marker
AB值：
0.473464
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
