典型文献
Cdx1b protects intestinal cell fate by repressing signaling networks for liver specification
文献摘要：
In mammals,the expression of the homeobox family member Cdx2/CDX2 is restricted within the intestine.Conditional ablation of the mouse Cdx2 in the endodermal cells causes a homeotic transformation of the intestine towards the esophagus or gastric fate.In this report,we show that null mutants of zebrafish cdx1b,encoding the counterpart of mammalian CDX2,could survive more than 10 days post fertilization,a stage when the zebrafish digestive system has been well developed.Through RNA sequencing(RNA-seq)and single-cell sequencing(scRNA-seq)of the dissected intestine from the mutant embryos,we demonstrate that the loss-of-function of the zebrafish cdx1b yields hepatocyte-like intestinal cells,a phenotype never observed in the mouse model.Further RNA-seq data analysis,and genetic double mutants and signaling inhibitor studies reveal that Cdx1b functions to guard the intestinal fate by repressing,directly or indirectly,a range of transcriptional factors and signaling pathways for liver specification.Finally,we demonstrate that heat shock-induced overexpression of cdx1b in a transgenic fish abolishes the liver formation.Therefore,we demonstrate that Cdx1b is a key repressor of hepatic fate during the intestine specification in zebrafish.
文献关键词：
中图分类号：
作者姓名：
Qingxia Jin;Yuqi Gao;Shimin Shuai;Yayue Chen;Kaiyuan Wang;Jun Chen;Jinrong Peng;Ce Gao
作者机构：
College of Animal Sciences,Zhejiang University,Hangzhou,Zhejiang 310058,China;Department of Human Cell Biology and Genetics,School of Medicine,Southern University of Science and Technology,Shenzhen,Guangdong 518055,China;College of Life Sciences,Zhejiang University,Hangzhou,Zhejiang 310058,China
文献出处：
引用格式：
[1]Qingxia Jin;Yuqi Gao;Shimin Shuai;Yayue Chen;Kaiyuan Wang;Jun Chen;Jinrong Peng;Ce Gao-.Cdx1b protects intestinal cell fate by repressing signaling networks for liver specification)[J].遗传学报,2022(12):1101-1113
A类：
Cdx1b,endodermal,cdx1b
B类：
protects,intestinal,fate,by,repressing,signaling,networks,liver,specification,In,mammals,homeobox,family,member,Cdx2,CDX2,restricted,within,intestine,Conditional,ablation,mouse,cells,causes,homeotic,transformation,towards,esophagus,gastric,this,report,show,that,null,mutants,zebrafish,encoding,counterpart,mammalian,could,survive,more,than,days,post,fertilization,stage,when,digestive,system,has,been,well,developed,Through,sequencing,single,scRNA,dissected,from,embryos,demonstrate,loss,yields,hepatocyte,like,phenotype,never,observed,model,Further,data,analysis,genetic,double,inhibitor,studies,reveal,functions,guard,indirectly,range,transcriptional,factors,pathways,Finally,heat,shock,induced,overexpression,transgenic,abolishes,Therefore,key,repressor,hepatic,during
AB值：
0.54618
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