Rice OsLIC encoding a CCCH zinc finger tran-scription factor plays an important role in immunity. However, the immune signaling pathways that OsLIC-involved and the underlying mechanisms that OsLIC-conferred resistance against pathogens are largely unclear. Here, we show that OsLIC, as a substrate for OsMAPK6, negatively regulates re-sistance to Xanthomonas oryzae pv. oryzae (Xoo) and X. oryzae pv. oryzicola (Xoc) by directly sup-pressing OsWRKY30 transcription. Biochemical assays showed that OsLIC bound to OsWRKY30 promoter and suppressed its transcription. Genetic assays confirmed that the osilc knockout mutants and OsWRKY30-overexpressing plants exhibited enhanced resistance to Xoo and Xoc, knocking out OsWRKY30 in the oslic mutants attenuated the re-sistance against bacterial pathogens. OsMAPK6 physically interacted with and phosphorylated OsLIC leading to decreased OsLIC DNA-binding activity, therefore, overexpression of OsLIC parti-ally suppressed OsMAPK6-mediated rice resist-ance. In addition, both OsMAPK6-phosphorylated activation of OsLIC and phosphorylation-mimic OsLIC5D had reduced DNA-binding activity to-wards OsWRKY30 promoter, thereby promoting OsWRKY30 transcription. Collectively, these results reveal that OsMAPK6-mediated phosphor-ylation of OsLIC positively regulates rice resistance to Xoo and Xoc by modulating OsWRKY30 transcription, suggesting that OsMAPK6-OsLIC-OsWRKY30 module is an immune signaling pathway in response to the bacterial pathogens.

Lihan Wang;Jie Chen;Yuqin Zhao;Shiping Wang;Meng Yuan

National Key Laboratory of Crop Genetic Improvement,National Center of Plant Gene Research(Wuhan),Hubei Hongshan Laboratory,Huazhong Agricultural University,Wuhan 430070,China

植物学报（英文版）

[1]Lihan Wang;Jie Chen;Yuqin Zhao;Shiping Wang;Meng Yuan-.OsMAPK6 phosphorylates a zinc finger protein OsLIC to promote downstream OsWRKY30 for rice resistance to bacterial blight and leaf streak)[J].植物学报（英文版）,2022(05):1116-1130

OsMAPK6,OsLIC,OsWRKY30,Xoc,osilc,oslic,OsLIC5D

phosphorylates,zinc,finger,protein,downstream,rice,resistance,bacterial,blight,leaf,streak,Rice,encoding,CCCH,plays,important,role,immunity,However,immune,signaling,pathways,that,involved,underlying,mechanisms,conferred,against,pathogens,are,largely,unclear,Here,substrate,negatively,regulates,Xanthomonas,oryzae,pv,Xoo,oryzicola,directly,transcription,Biochemical,assays,showed,bound,promoter,suppressed,its,Genetic,confirmed,knockout,mutants,overexpressing,plants,exhibited,enhanced,knocking,attenuated,physically,interacted,phosphorylated,leading,decreased,binding,activity,therefore,overexpression,parti,mediated,In,addition,both,activation,phosphorylation,mimic,had,reduced,wards,thereby,promoting,Collectively,these,results,reveal,positively,modulating,suggesting,module,response

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