典型文献
Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon α2b
文献摘要：
Recombinant human interferon α2b(rhIFNα2b)is widely used as an antiviral therapy agent for the treatment of hepatitis B and hepatitis C.The current identification test for rhIFNα2b is complex.In this study,an anti-rhIFNα2b nanobody was discovered and used for the development of a rapid lateral flow strip for the identification of rhIFNα2b.RhIFNα2b was used to immunize an alpaca,which established a phage nanobody library.After five steps of enrichment,the nanobody I22,which specifically bound rhIFNα2b,was isolated and inserted into the prokaryotic expression vector pET28a.After subsequent purification,the physicochemical properties of the nanobody were determined.A semiquantitative detection and rapid identification assay of rhIFNα2b was developed using this novel nanobody.To develop a rapid test,the nanobody I22 was coupled with a colloidal gold to produce lateral-flow test strips.The developed rhIFNα2b detection assay had a limit of detection of 1 μg/mL.The isolation of I22 and successful construction of a lateral-flow immunochromatographic test strip demonstrated the feasibility of performing ligand-binding assays on a lateral-flow test strip using recombinant protein products.The principle of this novel assay is generally applicable for the rapid testing of other com-mercial products,with a great potential for routine use in detecting counterfeit recombinant protein products.
文献关键词：
中图分类号：
作者姓名：
Xi Qin;Maoqin Duan;Dening Pei;Jian Lin;Lan Wang;Peng Zhou;Wenrong Yao;Ying Guo;Xiang Li;Lei Tao;Youxue Ding;Lan Liu;Yong Zhou;Chuncui Jia;Chunming Rao;Junzhi Wang
作者机构：
National Institutes for Food and Drug Control,Beijing,100050,China;Synthetic and Functional Biomolecules Center,Peking University,Beijing,100871,China;WHO Collaboration Centre for Biologicals Standardization and Evaluation,Beijing,100050,China
文献出处：
引用格式：
[1]Xi Qin;Maoqin Duan;Dening Pei;Jian Lin;Lan Wang;Peng Zhou;Wenrong Yao;Ying Guo;Xiang Li;Lei Tao;Youxue Ding;Lan Liu;Yong Zhou;Chuncui Jia;Chunming Rao;Junzhi Wang-.Development of novel-nanobody-based lateral-flow immunochromatographic strip test for rapid detection of recombinant human interferon α2b)[J].药物分析学报（英文）,2022(02):308-316
A类：
RhIFN,alpaca,I22,counterfeit
B类：
Development,novel,nanobody,lateral,flow,immunochromatographic,rapid,detection,recombinant,human,interferon,2b,Recombinant,rhIFN,widely,used,antiviral,therapy,agent,treatment,hepatitis,current,identification,complex,In,this,study,was,discovered,development,immunize,which,established,phage,library,After,five,steps,enrichment,specifically,bound,isolated,inserted,into,prokaryotic,expression,vector,pET28a,subsequent,purification,physicochemical,properties,were,determined,semiquantitative,developed,using,To,coupled,colloidal,gold,produce,strips,had,limit,isolation,successful,construction,demonstrated,feasibility,performing,ligand,binding,assays,protein,products,principle,generally,applicable,testing,other,mercial,great,potential,routine,detecting
AB值：
0.428095
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