Weaning stress can cause tight junctions damage and intestinal permeability enhancement,which leads to intestinal imbalance and growth retardation,thereby causing damage to piglet growth and devel-opment.Spermine can reduce stress.However,the mechanism of spermine modulating the intestinal integrity in pigs remains largely unknown.This study aims to examine whether spermine protects the intestinal barrier integrity of piglets through ras-related C3 botulinum toxin substrate 1(Rac1)/phos-pholipase C-γ1(PLC-γ1)signaling pathway.In vivo,80 piglets were categorised into 4 control groups and 4 spermine groups(10 piglets per group).The piglets were fed with normal saline or spermine at 0.4 mmol/kg BW for 7 h and 3,6 and 9 d.In vitro,we investigated whether spermine protects the in-testinal barrier after a tumor necrosis factor α(TNF-α)challenge through Rac1/PLC-γ1 signaling pathway.The in vivo study found that spermine supplementation increased tight junction protein mRNA levels and Rac1/PLC-γ1 signaling pathway gene expression in the jejunum of piglets.The serum D-lactate content was significantly decreased after spermine supplementation(P＜0.05).The in vitro study found that 0.1 μmol/L spermine increased the levels of tight junction protein expression,Rac1/PLC-γ1 signaling pathway and transepithelial electrical resistance,and decreased paracellular permeability(P＜0.05).Further experiments demonstrated that spermine supplementation enhanced the levels of tight junction protein expression,Rac1/PLC-γ1 signaling pathway and transepithelial electrical resistance,and decreased paracellular permeability compared with the NSC-23766 and U73122 treatment with sper-mine after TNF-α challenge(P＜0.05).Collectively,spermine protects intestinal barrier integrity through Rac1/PLC-γ1 signaling pathway in piglets.

Guangmang Liu;Xiaomei Xu;Caimei Wu;Gang Jia;Hua Zhao;Xiaoling Chen;Gang Tian;Jingyi Cai;Jing Wang

Institute of Animal Nutrition,Sichuan Agricultural University,Chengdu,611130,China;Key Laboratory for Animal Disease-Resistance Nutrition of China Ministry of Education,Chengdu,611130,China;Maize Research Institute,Sichuan Agricultural University,Chengdu,611130,China

动物营养（英文）

[1]Guangmang Liu;Xiaomei Xu;Caimei Wu;Gang Jia;Hua Zhao;Xiaoling Chen;Gang Tian;Jingyi Cai;Jing Wang-.Spermine protects intestinal barrier integrity through ras-related C3 botulinum toxin substrate 1/phospholipase C-γ1 signaling pathway in piglets)[J].动物营养（英文）,2022(01):135-143

Spermine,Weaning,pholipase

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