Background:The emergence of antimicrobial resistance has necessitated the development of effective alternatives to antibiotics for livestock and poultry production. This study investigated a possible synergy between butyrate and forskolin (a natural labdane diterpene) in enhancing innate host defense, barrier function, disease resistance, growth performance, and meat quality of broilers. Methods:The expressions of representative genes involved in host defense (AvBD9 and AvBD10), barrier function (MUC2, CLDN1, and TJP1), and inflammation (IL-1β) were measured in chicken HD11 macrophages in response to butyrate and forskolin in the presence or absence of bacterial lipopolysaccharides (LPS). Intestinal lesions and the Clostridium perfringens titers were also assessed in C. perfringens-challenged chickens fed butyrate and forskolin- containing Coleus forskohlii (CF) extract individually or in combination. Furthermore, growth performance and carcass characteristics were evaluated in broilers supplemented with butyrate and the CF extract for 42 d. Results:Butyrate and forskolin synergistically induced the expressions of AvBD9, AvBD10, and MUC2 in chicken HD11 cells (P<0.05) and the synergy was maintained in the presence of LPS. Butyrate and forskolin also suppressed LPS-induced IL-1βgene expression in HD11 cells in a synergistic manner (P<0.05). The two compounds significantly reduced the intestinal lesions of C. perfringens-challenged chickens when combined (P<0.05), but not individually. Furthermore, butyrate in combination with forskolin-containing CF extract had no influence on weight gain, but significantly reduced feed intake (P<0.05) with a strong tendency to improve feed efficiency (P=0.07) in a 42-d feeding trial. Desirably, the butyrate/forskolin combination significantly decreased abdominal fat deposition (P=0.01) with no impact on the carcass yield, breast meat color, drip loss, or pH of d-42 broilers. Conclusions:Butyrate and forskolin has potential to be developed as novel antibiotic alternatives to improve disease resistance, feed efficiency, and carcass composition of broilers.

Qing Yang;Binlong Chen;Kelsy Robinson;Thiago Belem;Wentao Lyu;Zhuo Deng;Ranjith Ramanathan;Guolong Zhang

Department of Animal and Food Sciences,Oklahoma State University, Stillwater,OK,USA;College of Animal Science,Xichang University,Xichang,Sichuan,China;Present address:Poultry Production and Product Safety Research Unit,USDA–Agricultural Research Service(ARS),Fayetteville,AR, USA;State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-products,Institute of Agro-product Safety and Nutrition,Zhejiang Academy of Agricultural Sciences,Hangzhou,China;Center for Excellence in Hip Disorders,Texas Scottish Rite Hospital for Children,Dallas,TX,USA

畜牧与生物技术杂志（英文版）

[1]Qing Yang;Binlong Chen;Kelsy Robinson;Thiago Belem;Wentao Lyu;Zhuo Deng;Ranjith Ramanathan;Guolong Zhang-.Butyrate in combination with forskolin alleviates necrotic enteritis, increases feed efficiency, and improves carcass composition of broilers)[J].畜牧与生物技术杂志（英文版）,2022(04):1115-1125

Butyrate,labdane,AvBD9,AvBD10,Coleus,forskohlii,Desirably

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