This study was conducted to investigate the effects of glycerol monolaurate(GML)on lipopolysaccharide(LPS)-induced immunological stress and intestinal mucosal injury in broilers and its underlying mech-anisms.A total of 144 one-d-old Arbor Acres broilers were allocated to a 2×2 factorial arrangement involving dietary treatment(0 or 1,200 mg/kg dietary GML)and LPS challenge(injected with saline or Escherichia coli LPS on d 16,18,and 20).Samples were collected on d 21.The results revealed that dietary GML augmented serum immunoglobulin A(P=0.009)and immunoglobulin G(P＜0.001)levels in challenged birds.Dietary GML normalized LPS-induced variations in serum interleukin-6,interferon-gamma,and LPS levels(P＜0.05),jejunal villus height(P=0.030),and gene expression of interleukin-6,macrophage inflammatory protein-3 alpha,Toll-like receptor 4,nuclear factor kappa-B,caspase-1,tight junction proteins,adenosine monophosphate-activated protein kinase alpha 1(AMPKα1),nuclear factor-erythroid 2-related factor 2(Nrf2),and superoxide dismutase-1(P＜0.05).GML supplementation ameliorated LPS-induced peroxidation by reducing malondialdehyde content and increasing antioxidant enzyme activity(P＜0.05).Dietary GML enhanced the abundances of Anaerostipes,Pseudoflavonifractor,and Gordonibacter and reduced the proportion of Phascolarctobacterium in challenged birds.Dietary GML was positively correlated with alterations in antioxidant enzyme activities and AMPKα1,Nrf2,and zonula occludens-1 expressions.The genera Anaerostipes,Lachnospira,Gordonibacter,Lachnospira,Marvin-bryantia,Peptococcus,and Pseudoflavonifractor were linked to attenuated inflammation and improved antioxidant capacity of challenged birds.In conclusion,dietary GML alleviated LPS-induced immuno-logical stress and intestinal injury of broilers by suppressing inflammation and oxidative stress.Dietary GML regulated cecal microbiota and activated the AMPK/Nrf2 pathway in LPS-challenged broilers.

Linglian Kong;Zhenhua Wang;Chuanpi Xiao;Qidong Zhu;Zhigang Song

Department of Animal Science and Technology,Shandong Agricultural University,Tai'an,Shandong 271018,China;Center for Mitochondria and Healthy Ageing,College of Life Sciences,Yantai University,Yantai,Shandong 264005,China;Precision Livestock and Nutrition Unit,Gembloux Agro-Bio Tech,University of Liège,Gembloux 5030,Belgium

动物营养（英文）

[1]Linglian Kong;Zhenhua Wang;Chuanpi Xiao;Qidong Zhu;Zhigang Song-.Glycerol monolaurate attenuated immunological stress and intestinal mucosal injury by regulating the gut microbiota and activating AMPK/Nrf2 signaling pathway in lipopolysaccharide-challenged broilers)[J].动物营养（英文）,2022(03):347-359

Pseudoflavonifractor,Gordonibacter,Marvin,bryantia

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