Background:This study evaluated the effects of supplemental xylanase and xylooligosaccharides (XOS) in a corn-soybean meal (SBM)-based diet on growth performance and intestinal health of broilers. A total of 288 day-old chicks (Cobb 500) were allocated to 36 floor pens (8 birds/pen) equally in 9 dietary treatments in a 3 × 3 factorial arrangement. The treatments were combinations of 3 levels of xylanase (0, 0.005％and 0.01％Econase XT) and 3 levels of prebiotics (0, 0.005％and 0.01％XOS) added to basal mash diets formulated in three phases (starter, d 0–14;grower, d 15–28;finisher, d 29–42). The feed intake and body weights were recorded weekly. On d 42, ileal sections were collected for histomorphometric and gene expression analysis, and cecal content was collected for determining short-chain fatty acids (SCFA) and microbiota. Results:Xylanase linearly (P<0.01) increased the average daily gain (ADG) in both the finisher and total period and the final body weight gain (FBWG, 2940&2932 vs. 2760 g) of broilers. XOS did not significantly increase either ADG or FBWG (P>0.05). Supplemental xylanase and XOS did not affect average daily feed intake and feed conversion ratio (P>0.05). Xylanase and XOS did not change villus height (VH) or crypt depth (CD) ratio (P>0.05). However, xylanase exhibited a trend (P=0.097) on VH:CD ratio. The inclusion of 0.01％XOS without xylanase increased the level of IL-10 (a marker of anti-inflammatory cytokine) and IL-4 (a T-cell differentiation cytokine) genes compared with control (P<0.05). The acetate production was increased by xylanase (P<0.01) and XOS (P<0.05) without an additive effect. Xylanase increased total SCFA (P<0.01) while XOS had a tendency to increase (P=0.052). Alpha and beta diversity of microbiota among treatments were not different (P>0.05). However, the mean proportion of family Ruminococcaceae was increased by the supplemental 0.01％xylanase (P<0.01). Conclusion:It can be concluded that XOS can enhance cecal fermentation, while xylanase can increase the body weight gain along with the fermentation metabolites in the ceca of broilers fed the corn-SBM-based diet but the effects may not always translate into an improved mucosal absorptive capacity and a better feed efficiency.

Amit K.Singh;Birendra Mishra;Michael R.Bedford;Rajesh Jha

Department of Human Nutrition,Food and Animal Sciences,University of Hawaii at Manoa,Honolulu,HI 96822,USA;AB Vista Feed Ingredients,Marlborough,Wiltshire,UK

畜牧与生物技术杂志（英文版）

[1]Amit K.Singh;Birendra Mishra;Michael R.Bedford;Rajesh Jha-.Effects of supplemental xylanase and xylooligosaccharides on production performance and gut health variables of broiler chickens)[J].畜牧与生物技术杂志（英文版）,2022(01):166-180

Econase,finisher,histomorphometric,FBWG,ceca

Effects,supplemental,xylanase,xylooligosaccharides,production,performance,gut,health,variables,chickens,Background,This,study,evaluated,effects,XOS,corn,soybean,meal,SBM,growth,intestinal,broilers,total,day,old,chicks,Cobb,were,allocated,floor,pens,birds,equally,dietary,treatments,factorial,arrangement,combinations,levels,XT,prebiotics,added,basal,mash,diets,formulated,three,phases,starter,grower,feed,intake,body,weights,recorded,weekly,On,ileal,sections,collected,expression,analysis,cecal,content,was,determining,short,chain,fatty,acids,SCFA,microbiota,Results,Xylanase,linearly,increased,average,daily,gain,ADG,both,period,final,did,not,significantly,either,Supplemental,affect,conversion,ratio,change,villus,height,VH,crypt,depth,CD,However,exhibited,trend,inclusion,without,marker,anti,inflammatory,cytokine,cell,differentiation,genes,compared,control,acetate,by,additive,while,had,tendency,Alpha,beta,diversity,among,mean,proportion,family,Ruminococcaceae,Conclusion,It,concluded,that,enhance,fermentation,along,metabolites,fed,but,may,always,translate,into,improved,mucosal,absorptive,capacity,better,efficiency

0.510506