Weaning stress may cause reduced energy intake for maintenance of mucosal structure.Gln,Glu,and Asp are major energy sources for the small intestine.This study investigated whether Gln,Glu,and Asp improve the intestinal morphology via regulating the energy metabolism in weaning piglets.A total of 198 weaned piglets were assigned to 3 treatments:Control(Basal diet+1.59％L-Ala);T1(Basal diet+1％L-Gln+0.5％L-Glu+0.1％L-Asp);T2(Low energy diet+1％L-Gln+0.5％L-Glu+0.1％L-Asp).Jejunum and ileum were obtained on d 5 or 21 post-weaning.T1 enhanced growth performance.T1 and T2 treatments improved small intestinal morphology by increasing villus height,goblet cell number and decreasing crypt depth.Days post-weaning affected the efficacy of T2,but not T1,on energy metabolism.At normal energy supplementation,Gln,Glu,and Asp restored small intestinal energy homeostasis via replenishing the Krebs'cycle and down-regulating the AMPK(adenosine monophosphate activated protein kinase)pathway.As these are not sufficient to maintain the intestinal energy-balance of piglets fed with a low energy diet on d 5 post-weaning,the AMPK,glycolysis,beta-oxidation,and mitochondrial biogenesis are activated to meet the high energy demand of enterocytes.These data indicated that Gin,Glu,and Asp could restore the energy homeostasis of intestinal mucosa of weaning piglets under normal energy fed.Low energy feeding may increase the susceptibility of piglets to stress,which may decrease the efficacy of Gln,Glu,and Asp on the restoration of energy balance.These findings provide new in-formation on nutritional intervention for insufficient energy intake in weaning piglets.

College of Animal Science and Technology,Hunan Agricultural University,Changsha 410128,Hunan,China;Animal Nutrition and Human Health Laboratory,Hunan Provincial Key Laboratory of Animal Intestinal Function and Regulation,School of Life Sciences,Hunan Normal University,Changsha 410081,Hunan,China;Laboratory of Animal Nutritional Physiology and Metabolic Process,Key Laboratory of Agroecological Processes in Subtropical Region,National Engineering Laboratory for Pollution Control and Waste Utilization in Livestock and Poultry Production,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha 410125,Hunan,China;University of Chinese Academy of Sciences,Beijing 10008,China

[1]Jing Wang;Nan Wang;Ming Qi;Jianjun Li;Bie Tan-.Glutamine,glutamate,and aspartate differently modulate energy homeostasis of small intestine under normal or low energy status in piglets)[J].动物营养（英文）,2022(01):216-226

Weaning,diet+1,Gln+0,Glu+0,Jejunum

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