典型文献
CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant
文献摘要：
Coronavirus disease 2019(COVID-19)is a highly con-tagious disease caused by severe acute respiratory syndrome corona-virus 2(SARS-CoV-2).The gold standard method for the diagnosis of SARS-CoV-2 depends on quantitative reverse transcription-polymerase chain reaction till now,which is time-consuming and requires expen-sive instrumentation,and the confirmation of variants relies on further sequencing techniques.Herein,we first proposed a robust technique-methodology of electrochemical CRISPR sensing with the advantages of rapid,highly sensitivity and specificity for the detection of SARS-CoV-2 variant.To enhance the sensing capability,gold electrodes are uniformly decorated with electro-deposited gold nanoparticles.Using DNA template identical to SARS-CoV-2 Delta spike gene sequence as model,our biosensor exhibits excellent analytical detection limit(50 fM)and high linearity(R2=0.987)over six orders of magnitude dynamic range from 100 fM to 10 nM without any nucleic-acid-amplification assays.The detection can be completed within 1 h with high stability and specificity which benefits from the CRISPR-Cas system.Furthermore,based on the wireless micro-electrochemical platform,the proposed biosensor reveals promising application ability in point-of-care testing.
文献关键词：
中图分类号：
作者姓名：
Chenshuo Wu;Zhi Chen;Chaozhou Li;Yabin Hao;Yuxuan Tang;Yuxuan Yuan;Luxiao Chai;Taojian Fan;Jiangtian Yu;Xiaopeng Ma;Omar A.Al-Hartomy;S.Wageh;Abdullah G.Al-Sehemi;Zhiguang Luo;Yaqing He;Jingfeng Li;Zhongjian Xie;Han Zhang
作者机构：
International Collaborative Laboratory of 2D,Materials for Optoelectronics Science and Technology of Ministry of Education,Institute of Microscale Optoelectronics,College of Physics and Optoelectronic Engineering,Shenzhen University,Shenzhen 518060,People's Republic of China;Hospital of Guangzhou Medical University,Qingyuan city People's Hospital,Qingyuan 511518,People's Republic of China;Shenzhen Han's Tech Limited Company,Shenzhen 518000,People's Republic of China;Shenzhen International Institute for Biomedical Research,Shenzhen 518116,People's Republic of China;Department of Respiratory,Shenzhen Children's Hospital,Shenzhen 518038,People's Republic of China;Department of Physics,Faculty of Science,King Abdulaziz University,Jeddah 21589,Saudi Arabia;Research Center for Advanced Materials Science(RCAMS),King Khalid University,P.O.Box 9004,Abha 61413,Saudi Arabia;Department of Chemistry,College of Science,King Khalid University,P.O.Box 9004,Abha 61413,Saudi Arabia;Zhongmin(Shenzhen)Intelligent Ecology Co.,Ltd,Shenzhen 518055,People's Republic of China;Shenzhen Center for Disease Control and Prevention,Shenzhen 518055,People's Republic of China;Institute of Pediatrics,Shenzhen Children's Hospital,Shenzhen 518038,People's Republic of China
文献出处：
引用格式：
[1]Chenshuo Wu;Zhi Chen;Chaozhou Li;Yabin Hao;Yuxuan Tang;Yuxuan Yuan;Luxiao Chai;Taojian Fan;Jiangtian Yu;Xiaopeng Ma;Omar A.Al-Hartomy;S.Wageh;Abdullah G.Al-Sehemi;Zhiguang Luo;Yaqing He;Jingfeng Li;Zhongjian Xie;Han Zhang-.CRISPR-Cas12a-Empowered Electrochemical Biosensor for Rapid and Ultrasensitive Detection of SARS-CoV-2 Delta Variant)[J].纳微快报（英文）,2022(10):83-94
A类：
tagious,expen
B类：
CRISPR,Cas12a,Empowered,Electrochemical,Biosensor,Rapid,Ultrasensitive,Detection,SARS,CoV,Delta,Variant,Coronavirus,disease,highly,caused,by,severe,acute,respiratory,syndrome,corona,gold,standard,diagnosis,depends,quantitative,reverse,transcription,polymerase,chain,reaction,till,now,which,consuming,requires,sive,instrumentation,confirmation,variants,relies,further,sequencing,techniques,Herein,first,proposed,robust,methodology,electrochemical,sensing,advantages,rapid,sensitivity,specificity,detection,To,enhance,capability,electrodes,uniformly,decorated,deposited,nanoparticles,Using,template,identical,spike,gene,sequence,model,our,biosensor,exhibits,excellent,analytical,limit,fM,linearity,over,six,orders,magnitude,dynamic,range,from,nM,without,any,nucleic,acid,amplification,assays,can,completed,within,stability,benefits,system,Furthermore,wireless,micro,platform,reveals,promising,application,point,care,testing
AB值：
0.65414
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