典型文献
Visually Intracellular Detection of Telomerase Activity Based on DNA Strand Displacement Reaction and Gold Nanoparticle Labeling
文献摘要：
Telomerase,which is regarded as a common biomarker for early cancer diagnostics and a potential target for clinical therapies,has attracted considerable interests concerning its detection and monitoring.Herein,we propose a sensitive method by designing a gold nanoparticle(AuNP)probe for visually intracellular detection of telomerase activity.The AuNPs were functionalized with a telomer-ase substrate primer(SH-prime).A 6-carboxy-fluorescein(FAM)modified strand(FAM-probe)was attached to the surface of AuNP through its complementary stand(SH-attach).In the absence of telomerase,the fluorescence resonance energy transfer(FRET)from FAM to AuNPs results in efficient fluorescence quenching.In the presence of telomerase,SH-primers on AuNPs were extended with the repeat units(TTAGGGG)n.The extension sequence triggered the strand displacement of FAM-probe to restore the fluorescence signals.It is worth mentioning that the proposed strategy does not need to design complex hairpin structure and allows the meas-urement of telomerase in crude cell extracts down to 0.5 HeLa cells/μL in 2 h.In addition,the present sensing platform can be ap-plied to the visually intracellular detection of telomerase activity in living cells.
文献关键词：
中图分类号：
作者姓名：
Yajun Zhang;Shen Yan;Zhaoxin Chen;Xin Jiang;Shuang Rao;Zhuoran Jiang;Shanshan Qin;Xiang Zhou;Yuhao Du
作者机构：
College of Chemistry and Molecular Sciences,Key Laboratory of Biomedical Polymers of Ministry of Education,Wuhan University,Wuhan,Hubei 430072,China
文献出处：
引用格式：
[1]Yajun Zhang;Shen Yan;Zhaoxin Chen;Xin Jiang;Shuang Rao;Zhuoran Jiang;Shanshan Qin;Xiang Zhou;Yuhao Du-.Visually Intracellular Detection of Telomerase Activity Based on DNA Strand Displacement Reaction and Gold Nanoparticle Labeling)[J].中国化学（英文版）,2022(06):693-698
A类：
Visually,Telomerase,telomer,TTAGGGG
B类：
Intracellular,Detection,Activity,Based,Strand,Displacement,Reaction,Gold,Nanoparticle,Labeling,which,regarded,common,biomarker,early,cancer,diagnostics,potential,target,clinical,therapies,has,attracted,considerable,interests,concerning,detection,monitoring,Herein,sensitive,method,by,designing,gold,nanoparticle,probe,visually,intracellular,telomerase,activity,AuNPs,were,functionalized,substrate,SH,carboxy,fluorescein,FAM,modified,strand,was,attached,surface,through,complementary,stand,absence,fluorescence,resonance,energy,transfer,FRET,from,results,efficient,quenching,presence,primers,extended,repeat,units,extension,sequence,triggered,displacement,restore,signals,It,worth,mentioning,that,proposed,strategy,does,not,need,complex,hairpin,structure,allows,meas,urement,crude,extracts,down,HeLa,cells,addition,present,sensing,platform,plied,living
AB值：
0.585649
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