典型文献
A Vaccine Based on the Receptor-Binding Domain of the Spike Protein Expressed in Glycoengineered Pichia pastoris Targeting SARS-CoV-2 Stimulates Neutralizing and Protective Antibody Responses
文献摘要：
In 2020 and 2021,severe acute respiratory syndrome coronavirus 2(SARS-CoV-2),a novel coronavirus,caused a global pandemic.Vaccines are expected to reduce the pressure of prevention and control,and have become the most effective strategy to solve the pandemic crisis.SARS-CoV-2 infects the host by binding to the cellular receptor angiotensin converting enzyme 2(ACE2)via the receptor-binding domain(RBD)of the surface spike(S)glycoprotein.In this study,a candidate vaccine based on a RBD recombinant subunit was prepared by means of a novel glycoengineered yeast Pichia pastoris expression system with characteristics of glycosylation modification similar to those of mammalian cells.The candidate vaccine effectively stimulated mice to produce high-titer anti-RBD specific antibody.Furthermore,the specific antibody titer and virus-neutralizing antibody(NAb)titer induced by the vaccine were increased significantly by the combination of the double adjuvants Al(OH)3 and CpG.Our results showed that the virus-NAb lasted for more than six months in mice.To summarize,we have obtained a SARS-CoV-2 vaccine based on the RBD of the S glycoprotein expressed in glycoengineered Pichia pastoris,which stimulates neutralizing and protective antibody responses.A technical route for fucose-free complex-type N-glycosylation modified recombinant subunit vaccine preparation has been established.
文献关键词：
中图分类号：
作者姓名：
Bo Liu;Ying Yin;Yuxiao Liu;Tiantian Wang;Peng Sun;Yangqin Ou;Xin Gong;Xuchen Hou;Jun Zhang;Hongguang Ren;Shiqiang Luo;Qian Ke;Yongming Yao;Junjie Xu;Jun Wu
作者机构：
Department of Microorganism Engineering,Beijing Institute of Biotechnology,Beijing 100071,China;Medical Innovation Research Division&Fourth Medical Center of the Chinese PLA General Hospital,Beijing 100853,China;Department of Neurosurgery,First Medical Center of the Chinese PLA General Hospital,Beijing 100853,China;Shenzhen Taihe Biotechnology Co.Ltd.,Shenzhen 518001,China;Institute of Physical Science and Information Technology,Anhui University,Hefei 230000,China
文献出处：
引用格式：
[1]Bo Liu;Ying Yin;Yuxiao Liu;Tiantian Wang;Peng Sun;Yangqin Ou;Xin Gong;Xuchen Hou;Jun Zhang;Hongguang Ren;Shiqiang Luo;Qian Ke;Yongming Yao;Junjie Xu;Jun Wu-.A Vaccine Based on the Receptor-Binding Domain of the Spike Protein Expressed in Glycoengineered Pichia pastoris Targeting SARS-CoV-2 Stimulates Neutralizing and Protective Antibody Responses)[J].工程（英文）,2022(06):107-115
A类：
Glycoengineered,Stimulates,glycoengineered
B类：
Based,Receptor,Binding,Domain,Spike,Protein,Expressed,Pichia,pastoris,Targeting,SARS,CoV,Neutralizing,Protective,Antibody,Responses,In,severe,acute,respiratory,syndrome,coronavirus,novel,caused,global,pandemic,Vaccines,expected,reduce,pressure,prevention,control,have,become,most,strategy,solve,crisis,infects,host,by,binding,cellular,receptor,angiotensin,converting,enzyme,ACE2,via,domain,RBD,surface,spike,glycoprotein,this,study,candidate,vaccine,recombinant,subunit,was,prepared,means,yeast,expression,system,characteristics,glycosylation,modification,similar,those,mammalian,cells,effectively,stimulated,mice,produce,high,titer,specific,antibody,Furthermore,neutralizing,NAb,induced,were,increased,significantly,combination,double,adjuvants,CpG,Our,results,showed,that,lasted,than,six,months,To,summarize,obtained,expressed,which,stimulates,protective,responses,technical,route,fucose,free,complex,type,modified,preparation,has,been,established
AB值：
0.593308
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机标中图分类号，由域田数据科技根据网络公开资料自动分析生成，仅供学习研究参考。
