典型文献
Quantum Dot Nanobeads-Labelled Lateral Flow Immunoassay Strip for Rapid and Sensitive Detection of Salmonella Typhimurium Based on Strand Displacement Loop-Mediated Isothermal Amplification
文献摘要：
Rapid,sensitive,point-of-care detection of pathogenic bacteria is important for food safety.In this study,we developed a novel quantum dot nanobeads-labelled lateral flow immunoassay strip(QBs-labelled LFIAS)combined with strand displacement loop-mediated isothermal amplification(SD-LAMP)for quan-titative Salmonella Typhimurium(ST)detection.Quantum dot nanobeads(QBs)served as fluorescence reporters,providing good detection efficiency.The customizable strand displacement(SD)probe was used in LAMP to improve the specificity of the method and prevent by-product capture.Detection was based on a sandwich immunoassay.A fluorescence strip reader measured the fluorescence intensity(FI)of the test(T)line and control(C)line.The linear detection range of the strip was 102-108 colony forming units(CFU)·mL-1.The visual limit of detection was 103 CFU·mL-1,indicating that the system was ten-fold more sensitive than AuNPs-labelled test strips.ST specificity was analyzed in accordance with agarose gel outputs of polymerase chain reaction(PCR)and SD-LAMP.We detected ST in foods with an acceptable recovery of 85％-110％.The method is rapid,simple,almost equipment-free,and suitable for bacterial detection in foods and for clinical diagnosis.
文献关键词：
中图分类号：
作者姓名：
Yuting Shang;Shuzhen Cai;Qinghua Ye;Qingping Wu;Yanna Shao;Xiaoying Qu;Xinran Xiang;Baoqing Zhou;Yu Ding;Moutong Chen;Liang Xue;Honghui Zhu;Jumei Zhang
作者机构：
Guangdong Provincial Key Laboratory of Microbial Safety and Health&State Key Laboratory of Applied Microbiology Southern China&Institute of Microbiology,Guangdong Academy of Sciences,Guangzhou 510070,China;State Key Laboratory of Food Science and Technology&National Engineering Research Center for Functional Foods&Synergetic Innovation Center of Food Safety&Joint International Research Laboratory on Food Safety,School of Food Science and Technology,Jiangnan University,Wuxi 214122,China
文献出处：
引用格式：
[1]Yuting Shang;Shuzhen Cai;Qinghua Ye;Qingping Wu;Yanna Shao;Xiaoying Qu;Xinran Xiang;Baoqing Zhou;Yu Ding;Moutong Chen;Liang Xue;Honghui Zhu;Jumei Zhang-.Quantum Dot Nanobeads-Labelled Lateral Flow Immunoassay Strip for Rapid and Sensitive Detection of Salmonella Typhimurium Based on Strand Displacement Loop-Mediated Isothermal Amplification)[J].工程（英文）,2022(12):62-70
A类：
Nanobeads,Labelled,Immunoassay,nanobeads,QBs,LFIAS
B类：
Quantum,Dot,Lateral,Flow,Strip,Rapid,Sensitive,Detection,Salmonella,Typhimurium,Based,Strand,Displacement,Loop,Mediated,Isothermal,Amplification,sensitive,point,care,detection,pathogenic,important,safety,In,this,study,we,developed,novel,quantum,dot,labelled,lateral,flow,immunoassay,combined,strand,displacement,loop,mediated,isothermal,amplification,LAMP,titative,ST,served,fluorescence,reporters,providing,good,efficiency,customizable,probe,was,used,improve,specificity,method,prevent,by,product,capture,sandwich,reader,measured,intensity,test,control,linear,range,colony,forming,units,CFU,visual,limit,indicating,that,system,fold,more,than,AuNPs,strips,analyzed,accordance,agarose,gel,outputs,polymerase,chain,reaction,We,detected,foods,acceptable,recovery,rapid,simple,almost,equipment,free,suitable,bacterial,clinical,diagnosis
AB值：
0.567414
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