OBJECTIVE:To investigate the protective efficacy of Bushen Culuan decoction补肾促卵方,BCD)on ovarian follicle and follicular granulosa cells in mice with premature ovarian insufficiency(POI)induced by tripterygium wilfordii polyglycoside,and to study the potential mechanism underlying the action.METHODS:Eighty female Balb/c mice were randomly divided into 4 groups(n=20 each):blank group,model group,Bushen Culuan decoction intervening group(BCD group)and estradiol valerate intervening group(EV group).In the first 14 model establishing d,mice in model group,BCD group and EV group were under Tripterygium wilfordii polyglycoside(TWP)gavage to establish POI models.In the 14-day therapeutic stage,mice in BCD group were taken BCD 18.35 mg·kg-1·d-1,mice in EV group were taken EV solution 0.15 mg·kg-1·d-1,while mice in blank group and model group were taken normal saline.When the mice accomplished therapy,whole blood was collected for serum hormone including follicle stimulating hormone(FSH),luteal hormone(LH),estradiol(E2),anti-mullerian hormone(AMH)levels and vascular endothelial growth factor(VEGF),bone morphogenetic protein-7(BMP-7)measurement.Ovarian tissues were harvested for morphologic observation,follicle counting,ovarian follicular graulosa cell apoptosis test and testing BMP-7 and caspase-3 expressions.RESULTS:The body weights of the mice kept growing stably in the process expect in TWP intervening stage.Compared with model group,BCD group had significantly higher ovarian index,serum E2,AMH,VEGF,BMP-7 levels and significantly lower FSH level(P＜0.05).Meanwhile the VEGF level in BCD group was higher than in EV group(P＜0.05).Compared with model group,the histopathological damage and GCs apoptosis were mitigated;developing follicle counting,BMP-7 expression were up-regulated,and caspase-3 expression was down-regulated in BCD groups(P＜0.05).CONCLUSION:BCD treatment could attenuate pathological process in POI ovaries,suppress GC apoptosis,probably through promoting BMP-7 expression and following inhibiting caspase-3 activation.

MA Kun;YUAN Yuan;CHEN Yanxia;YUAN Bochao;WANG Kaili;TIAN Caidie;LI Jiani

the Department of Educational Management,China Academy of Chinese Medical Science,Beijing 100007,China;Xiyuan Hospital,China Academy of Chinese Medical Science,Beijing 100091,China

中医杂志（英文版）

[1]MA Kun;YUAN Yuan;CHEN Yanxia;YUAN Bochao;WANG Kaili;TIAN Caidie;LI Jiani-.Efficacy of Bushen Culuan decoction(补肾促卵方)on ovarian follicle and follicular granulosa cells in mice with premature ovarian insufficiency induced by tripterygium wilfordii polyglycoside)[J].中医杂志（英文版）,2022(01):23-29

Bushen,Culuan,polyglycoside,valerate,graulosa

Efficacy,decoction,补肾促卵方,ovarian,follicle,follicular,granulosa,cells,mice,premature,insufficiency,induced,by,tripterygium,wilfordii,OBJECTIVE,To,investigate,protective,efficacy,BCD,POI,study,potential,mechanism,underlying,action,METHODS,Eighty,female,Balb,were,randomly,divided,into,groups,each,blank,intervening,estradiol,EV,In,first,establishing,Tripterygium,TWP,gavage,models,day,therapeutic,stage,taken,solution,normal,saline,When,accomplished,therapy,whole,blood,was,collected,serum,hormone,including,stimulating,FSH,luteal,LH,E2,anti,mullerian,AMH,levels,vascular,endothelial,growth,VEGF,bone,morphogenetic,protein,BMP,measurement,Ovarian,tissues,harvested,morphologic,observation,counting,apoptosis,testing,caspase,expressions,RESULTS,body,weights,kept,growing,stably,process,expect,Compared,had,significantly,higher,lower,Meanwhile,than,histopathological,damage,GCs,mitigated,developing,regulated,down,CONCLUSION,treatment,could,attenuate,ovaries,suppress,probably,through,promoting,following,inhibiting,activation

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